PUBLICATION
foxm1 Modulates Cell Non-Autonomous Response in Zebrafish Skeletal Muscle Homeostasis
- Authors
- Ferreira, F.J., Carvalho, L., Logarinho, E., Bessa, J.
- ID
- ZDB-PUB-210603-23
- Date
- 2021
- Source
- Cells 10(5): (Other)
- Registered Authors
- Bessa, Jose, Ferreira, Fábio
- Keywords
- CRISPR/Cas9, foxm1, satellite cells, skeletal muscle, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- CRISPR-Associated Protein 9/genetics
- CRISPR-Associated Protein 9/metabolism
- CRISPR-Cas Systems
- Cell Death
- Cell Differentiation
- Cell Proliferation
- Clustered Regularly Interspaced Short Palindromic Repeats
- Forkhead Box Protein M1/genetics
- Forkhead Box Protein M1/metabolism*
- Gene Editing
- Gene Expression Regulation, Developmental
- Muscle Development*
- Muscle Fibers, Fast-Twitch/metabolism
- Muscle Fibers, Fast-Twitch/pathology
- Muscle, Skeletal/metabolism*
- Muscle, Skeletal/pathology
- Satellite Cells, Skeletal Muscle/metabolism
- Satellite Cells, Skeletal Muscle/pathology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 34070077 Full text @ Cells
Citation
Ferreira, F.J., Carvalho, L., Logarinho, E., Bessa, J. (2021) foxm1 Modulates Cell Non-Autonomous Response in Zebrafish Skeletal Muscle Homeostasis. Cells. 10(5).
Abstract
foxm1 is a master regulator of the cell cycle, contributing to cell proliferation. Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggesting non-proliferative functions that remain largely unexplored. In this study, we used CRISPR/Cas9 to disrupt foxm1 in the zebrafish terminally differentiated fast-twitching muscle cells. foxm1 genomic disruption increased myofiber death and clearance. Interestingly, this contributed to non-autonomous satellite cell activation and proliferation. Moreover, we observed that Cas9 expression alone was strongly deleterious to muscle cells. Our report shows that foxm1 modulates a muscle non-autonomous response to myofiber death and highlights underreported toxicity to high expression of Cas9 in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping