PUBLICATION
Toxic effects of octocrylene on zebrafish larvae and liver cell line (ZFL)
- Authors
- Meng, Q., Yeung, K., Chan, K.M.
- ID
- ZDB-PUB-210520-11
- Date
- 2021
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 236: 105843 (Journal)
- Registered Authors
- Chan, King-Ming
- Keywords
- Endocrine disruption, Gene transcription, RNA-Seq, Sexual differentiation, Sunscreen chemicals
- MeSH Terms
-
- Acrylates/toxicity*
- Animals
- Cell Line
- Estrogens/metabolism
- Hepatocytes/drug effects
- Humans
- Larva/drug effects
- Lethal Dose 50
- Liver/drug effects
- Sunscreening Agents/toxicity
- Transcriptome
- Vitellogenins/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- PubMed
- 34010734 Full text @ Aquat. Toxicol.
Citation
Meng, Q., Yeung, K., Chan, K.M. (2021) Toxic effects of octocrylene on zebrafish larvae and liver cell line (ZFL). Aquatic toxicology (Amsterdam, Netherlands). 236:105843.
Abstract
Octocrylene (OC) is a broad-spectrum ultraviolet-absorbing chemical used in sunscreen and other personal care products. Its health effects are a concern because it has been detected in water, fish, humans, and food chains. In vivo and in vitro investigations were performed in zebrafish (Danio rerio) larvae and a zebrafish liver cell line (ZFL), respectively, to understand the potential risks and molecular mechanisms of OC toxicity. The 96-h median lethal concentration (LC50) of OC was determined to be 251.8 μM in larvae and 5.5 μM in ZFL cells. Quantitative real-time PCR (qRT-PCR) showed that OC induced the expression of genes for CYPs (CYP1A, CYP3A65), estrogen receptors (ERα, ERβ1, GPER), vitellogenin (VTG1), and sex determination (BRCA2, CYP19A, DMRT1, SOX9A), both in vitro and in vivo. A whole-transcriptome sequencing method was used to evaluate the gene expression profile of larvae exposed to OC. OC was found to mediate the biosynthesis of estrogens (such as estriol) and affect the antioxidant pathway (glutathione transferases and peroxisome). These findings clarify the toxic effects and molecular mechanisms of OC and support banning its use in cosmetics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping