PUBLICATION
EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia
- Authors
- Kindt, K.S., Akturk, A., Jarysta, A., Day, M., Beirl, A., Flonard, M., Tarchini, B.
- ID
- ZDB-PUB-210519-24
- Date
- 2021
- Source
- Nature communications 12: 2861 (Journal)
- Registered Authors
- Beirl, Alisha, Kindt, Katie
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Polarity/genetics
- Epithelium/metabolism*
- Female
- GTP-Binding Protein alpha Subunits, Gi-Go/genetics
- GTP-Binding Protein alpha Subunits, Gi-Go/metabolism*
- Hair Cells, Auditory/cytology
- Hair Cells, Auditory/metabolism*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Male
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Microscopy, Confocal/methods
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish/metabolism
- PubMed
- 34001891 Full text @ Nat. Commun.
Citation
Kindt, K.S., Akturk, A., Jarysta, A., Day, M., Beirl, A., Flonard, M., Tarchini, B. (2021) EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia. Nature communications. 12:2861.
Abstract
Hair cells detect sound, head position or water movements when their mechanosensory hair bundle is deflected. Each hair bundle has an asymmetric architecture that restricts stimulus detection to a single axis. Coordinated hair cell orientations within sensory epithelia further tune stimulus detection at the organ level. Here, we identify GPR156, an orphan GPCR of unknown function, as a critical regulator of hair cell orientation. We demonstrate that the transcription factor EMX2 polarizes GPR156 distribution, enabling it to signal through Gαi and trigger a 180° reversal in hair cell orientation. GPR156-Gαi mediated reversal is essential to establish hair cells with mirror-image orientations in mouse otolith organs in the vestibular system and in zebrafish lateral line. Remarkably, GPR156-Gαi also instructs hair cell reversal in the auditory epithelium, despite a lack of mirror-image organization. Overall, our work demonstrates that conserved GPR156-Gαi signaling is integral to the framework that builds directional responses into mechanosensory epithelia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping