PUBLICATION
Generation of a Novel Transgenic Zebrafish for Studying Adipocyte Development and Metabolic Control
- Authors
- Mao, Y., Hong, K.H., Liao, W., Li, L., Kim, S.J., Xiong, Y., Nam, I.K., Choe, S.K., Kwak, S.A.
- ID
- ZDB-PUB-210501-32
- Date
- 2021
- Source
- International Journal of Molecular Sciences 22(8): (Journal)
- Registered Authors
- Choe, Seong-Kyu
- Keywords
- adipose tissue, live imaging, obesity, transgenic zebrafish
- MeSH Terms
-
- Adipocytes/cytology*
- Adipocytes/metabolism*
- Adipose Tissue/metabolism
- Animal Nutritional Physiological Phenomena
- Animals
- Animals, Genetically Modified
- Cell Shape
- Green Fluorescent Proteins/metabolism
- Larva/genetics
- Larva/metabolism
- Promoter Regions, Genetic/genetics
- Transgenes
- Zebrafish/genetics*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 33924375 Full text @ Int. J. Mol. Sci.
Citation
Mao, Y., Hong, K.H., Liao, W., Li, L., Kim, S.J., Xiong, Y., Nam, I.K., Choe, S.K., Kwak, S.A. (2021) Generation of a Novel Transgenic Zebrafish for Studying Adipocyte Development and Metabolic Control. International Journal of Molecular Sciences. 22(8):.
Abstract
Zebrafish have become a popular animal model for studying various biological processes and human diseases. The metabolic pathways and players conserved among zebrafish and mammals facilitate the use of zebrafish to understand the pathological mechanisms underlying various metabolic disorders in humans. Adipocytes play an important role in metabolic homeostasis, and zebrafish adipocytes have been characterized. However, a versatile and reliable zebrafish model for long-term monitoring of adipose tissues has not been reported. In this study, we generated stable transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) in adipocytes. The transgenic zebrafish harbored adipose tissues that could be detected using GFP fluorescence and the morphology of single adipocyte could be investigated in vivo. In addition, we demonstrated the applicability of this model to the long-term in vivo imaging of adipose tissue development and regulation based on nutrition. The transgenic zebrafish established in this study may serve as an excellent tool to advance the characterization of white adipose tissue in zebrafish, thereby aiding the development of therapeutic interventions to treat metabolic diseases in humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping