PUBLICATION

Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5

Authors
Jacinto, R., Sampaio, P., Roxo-Rosa, M., Pestana, S., Lopes, S.S.
ID
ZDB-PUB-210420-7
Date
2021
Source
Frontiers in cell and developmental biology   9: 624531 (Journal)
Registered Authors
Lopes, Susana
Keywords
Pkd2, cilia length, dand5, flow dynamics, left-right, zebrafish
MeSH Terms
none
PubMed
33869175 Full text @ Front Cell Dev Biol
Abstract
The left-right (LR) field recognizes the importance of the mechanism involving the calcium permeable channel Polycystin-2. However, whether the early LR symmetry breaking mechanism is exclusively via Polycystin-2 has not been tested. For that purpose, we need to be able to isolate the effects of decreasing the levels of Pkd2 protein from any eventual effects on flow dynamics. Here we demonstrate that curly-up (cup) homozygous mutants have abnormal flow dynamics. In addition, we performed one cell stage Pkd2 knockdowns and LR organizer specific Pkd2 knockdowns and observed that both techniques resulted in shorter cilia length and abnormal flow dynamics. We conclude that Pkd2 reduction leads to LR defects that cannot be assigned exclusively to its putative role in mediating mechanosensation because indirectly, by modifying cell shape or decreasing cilia length, Pkd2 deficit affects LR flow dynamics.
Genes / Markers
Figures
Figure Gallery (3 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tc321
    		Point Mutation
    zf417TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      dnah7MO1-dnah7MRPHLNO
      pkd2MO3-pkd2MRPHLNO
      1 - 2 of 2
      Show
      Fish
      No data available
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
      No data available
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Jacinto, R., Sampaio, P., Roxo-Rosa, M., Pestana, S., Lopes, S.S. (2021) Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5. Frontiers in cell and developmental biology. 9:624531.