PUBLICATION

Ground state depletion microscopy as a tool for studying microglia-synapse interactions

Authors
Paasila, P.J., Fok, S.Y.Y., Flores-Rodriguez, N., Sajjan, S., Svahn, A.J., Dennis, C.V., Holsinger, R.M.D., Kril, J.J., Becker, T.S., Banati, R.B., Sutherland, G.T., Graeber, M.B.
ID
ZDB-PUB-210309-11
Date
2021
Source
Journal of neuroscience research   99(6): 1515-1532 (Journal)
Registered Authors
Becker, Thomas S., Svahn, Adam
Keywords
Alzheimer disease, RRID:AB_141874, RRID:AB_2199013, RRID:AB_2286948, RRID:AB_2534072, RRID:AB_2534076, RRID:AB_2535731, RRID:AB_2536183, RRID:AB_2633277, RRID:AB_324660, RRID:AB_839504, RRID:SCR_001622, RRID:SCR_002285, RRID:SCR_002798, RRID:SCR_013673, RRID:SCR_013726, ground state depletion followed by individual molecule return microscopy, microglia, post-mortem archival human brain tissue, super-resolution microscopy, synapses, zebrafish
MeSH Terms
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease/pathology
  • Animals
  • Antibodies
  • Female
  • Humans
  • Larva
  • Male
  • Mice
  • Microglia/physiology*
  • Microglia/ultrastructure*
  • Microscopy/methods*
  • Microscopy, Confocal
  • Middle Aged
  • Presynaptic Terminals/physiology
  • Presynaptic Terminals/ultrastructure
  • Synapses/physiology*
  • Synapses/ultrastructure*
  • Tissue Fixation
  • Zebrafish
PubMed
33682204 Full text @ J. Neurosci. Res.
Abstract
Ground state depletion followed by individual molecule return microscopy (GSDIM) has been used in the past to study the nanoscale distribution of protein co-localization in living cells. We now demonstrate the successful application of GSDIM to archival human brain tissue sections including from Alzheimer's disease cases as well as experimental tissue samples from mouse and zebrafish larvae. Presynaptic terminals and microglia and their cell processes were visualized at a resolution beyond diffraction-limited light microscopy, allowing clearer insights into their interactions in situ. The procedure described here offers time and cost savings compared to electron microscopy and opens the spectrum of molecular imaging using antibodies and super-resolution microscopy to the analysis of routine formalin-fixed paraffin sections of archival human brain. The investigation of microglia-synapse interactions in dementia will be of special interest in this context.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping