PUBLICATION
Heparan sulfate proteoglycan expression in the regenerating zebrafish fin
- Authors
- Keil, S., Gupta, M., Brand, M., Knopf, F.
- ID
- ZDB-PUB-210228-4
- Date
- 2021
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 250(9): 1368-1380 (Journal)
- Registered Authors
- Brand, Michael, Gupta, Mansi, Knopf, Franziska
- Keywords
- Glypican, extracellular matrix protein, growth factor, syndecan
- MeSH Terms
-
- Animal Fins/metabolism
- Animals
- Hedgehog Proteins*/metabolism
- Heparan Sulfate Proteoglycans/genetics
- Heparan Sulfate Proteoglycans/metabolism
- Signal Transduction
- Zebrafish*
- PubMed
- 33638212 Full text @ Dev. Dyn.
Citation
Keil, S., Gupta, M., Brand, M., Knopf, F. (2021) Heparan sulfate proteoglycan expression in the regenerating zebrafish fin. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(9):1368-1380.
Abstract
Background HSPG expression is found in many animal tissues and regulates growth factor signaling such as of Fibroblast growth factors (Fgf), Wingless/Int (Wnt) and Hedgehog (HH). Glypicans, which are GPI (glycosylphosphatidylinositol)-anchored proteins, and transmembrane-anchored syndecans represent two major HSPG protein families whose involvement in development and disease has been demonstrated. Their participation in regenerative processes both of the central nervous system and of regenerating limbs is well documented. However, whether HSPG are expressed in regenerating zebrafish fins, is currently unknown.
Results Here, we carried out a systematic screen of glypican and syndecan mRNA expression in regenerating zebrafish fins during the outgrowth phase. We find that eight out of the ten zebrafish glypicans and the three known zebrafish syndecans show specific expression at three days post amputation. Expression is found in different domains of the regenerate, including the distal and lateral basal layers of the wound epidermis, the distal most blastema and more proximal blastema regions.
Conclusions HSPG expression is prevalent in regenerating zebrafish fins. Further research is needed to delineate the function of glypican and syndecan action during zebrafish fin regeneration. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping