PUBLICATION
Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos
- Authors
- Mishima, Y., Inoue, K.
- ID
- ZDB-PUB-210220-46
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2218: 347-354 (Chapter)
- Registered Authors
- Inoue, Kunio, Mishima, Yuichiro
- Keywords
- Post-transcriptional regulation, Tethering, Translation, Zebrafish, mRNA
- MeSH Terms
-
- Animals
- Biological Assay/methods*
- Embryo, Nonmammalian/physiology*
- RNA/genetics*
- RNA Processing, Post-Transcriptional/genetics
- RNA, Messenger/genetics
- RNA-Binding Proteins/genetics*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 33606244 Full text @ Meth. Mol. Biol.
Citation
Mishima, Y., Inoue, K. (2021) Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 2218:347-354.
Abstract
Many proteins are assumed to mediate post-transcriptional regulation of mRNAs. However, the lack of information about their target mRNAs and functional domains hampers the detailed analysis of their molecular function. Here we describe a method to analyze the post-transcriptional effects of proteins of interest by artificially tethering the protein to a reporter mRNA in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping