ZFIN ID: ZDB-PUB-210220-46
Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos
Mishima, Y., Inoue, K.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2218: 347-354 (Chapter)
Registered Authors: Inoue, Kunio, Mishima, Yuichiro
Keywords: Post-transcriptional regulation, Tethering, Translation, Zebrafish, mRNA
MeSH Terms:
  • Animals
  • Biological Assay/methods*
  • Embryo, Nonmammalian/physiology*
  • RNA/genetics*
  • RNA Processing, Post-Transcriptional/genetics
  • RNA, Messenger/genetics
  • RNA-Binding Proteins/genetics*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 33606244 Full text @ Meth. Mol. Biol.
Many proteins are assumed to mediate post-transcriptional regulation of mRNAs. However, the lack of information about their target mRNAs and functional domains hampers the detailed analysis of their molecular function. Here we describe a method to analyze the post-transcriptional effects of proteins of interest by artificially tethering the protein to a reporter mRNA in zebrafish embryos.