|ZFIN ID: ZDB-PUB-210220-46|
Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos
Mishima, Y., Inoue, K.
|Source:||Methods in molecular biology (Clifton, N.J.) 2218: 347-354 (Chapter)|
|Registered Authors:||Inoue, Kunio, Mishima, Yuichiro|
|Keywords:||Post-transcriptional regulation, Tethering, Translation, Zebrafish, mRNA|
|PubMed:||33606244 Full text @ Meth. Mol. Biol.|
Mishima, Y., Inoue, K. (2021) Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 2218:347-354.
ABSTRACTMany proteins are assumed to mediate post-transcriptional regulation of mRNAs. However, the lack of information about their target mRNAs and functional domains hampers the detailed analysis of their molecular function. Here we describe a method to analyze the post-transcriptional effects of proteins of interest by artificially tethering the protein to a reporter mRNA in zebrafish embryos.
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