PUBLICATION
Vitamin E Deficiency Disrupts Gene Expression Networks during Zebrafish Development
- Authors
- Head, B., Ramsey, S.A., Kioussi, C., Tanguay, R.L., Traber, M.G.
- ID
- ZDB-PUB-210213-10
- Date
- 2021
- Source
- Nutrients 13(2): (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- E+, VitE sufficient, E–, VitE deficient, VitE, hours post-fertilization, hpf, vitamin E, α-TTP, α-tocopherol transfer protein.
- Datasets
- GEO:GSE164848
- MeSH Terms
-
- Animals
- Blotting, Western
- Gene Expression Regulation, Developmental*
- Vitamin E Deficiency/embryology
- Vitamin E Deficiency/veterinary*
- Zebrafish/embryology
- Zebrafish/growth & development
- PubMed
- 33573233 Full text @ Nutrients
Citation
Head, B., Ramsey, S.A., Kioussi, C., Tanguay, R.L., Traber, M.G. (2021) Vitamin E Deficiency Disrupts Gene Expression Networks during Zebrafish Development. Nutrients. 13(2):.
Abstract
Vitamin E (VitE) is essential for vertebrate embryogenesis, but the mechanisms involved remain unknown. To study embryonic development, we fed zebrafish adults (>55 days) either VitE sufficient (E+) or deficient (E-) diets for >80 days, then the fish were spawned to generate E+ and E- embryos. To evaluate the transcriptional basis of the metabolic and phenotypic outcomes, E+ and E- embryos at 12, 18 and 24 h post-fertilization (hpf) were subjected to gene expression profiling by RNASeq. Hierarchical clustering, over-representation analyses and gene set enrichment analyses were performed with differentially expressed genes. E- embryos experienced overall disruption to gene expression associated with gene transcription, carbohydrate and energy metabolism, intracellular signaling and the formation of embryonic structures. mTOR was apparently a major controller of these changes. Thus, embryonic VitE deficiency results in genetic and transcriptional dysregulation as early as 12 hpf, leading to metabolic dysfunction and ultimately lethal outcomes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping