ZFIN ID: ZDB-PUB-201222-4
Nephroprotective Role of Zhibai Dihuang Wan in Aristolochic Acid-Intoxicated Zebrafish
Lu, P.H., Lee, H.Y., Liou, Y.L., Tung, S.F., Kuo, K.L., Chen, Y.H.
Date: 2020
Source: BioMed Research International   2020: 5204348 (Journal)
Registered Authors: Chen, Yau-Hung
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Aristolochic Acids/toxicity*
  • Drugs, Chinese Herbal/pharmacology*
  • Gene Expression Profiling
  • Green Fluorescent Proteins/metabolism
  • Immune System/drug effects
  • Kidney/drug effects*
  • Kidney Diseases/drug therapy*
  • Kidney Diseases/metabolism
  • Medicine, Chinese Traditional
  • Phenotype
  • Protective Agents/pharmacology*
  • Renal Insufficiency/chemically induced*
  • Sodium-Potassium-Exchanging ATPase/metabolism
  • Zebrafish
PubMed: 33344639 Full text @ Biomed Res. Int.
Zhibai Dihuang Wan (ZDW) is an eight-herbal formula of traditional Chinese medicine. Clinically, it regulated immune activity and was used to treat diabetes and renal disease. In this study, we aimed to explore the nephroprotective effect of ZDW in an aristolochic acid- (AA-) intoxicated zebrafish model. We used a green fluorescent kidney transgenic zebrafish to evaluate the nephroprotective effects of ZDW by recording subtle changes in the kidney. Our results demonstrated that ZDW treatment can attenuate AA-induced kidney malformations (60% for AA-treated, 47% for pretreatment with ZDW, and 17% for cotreatment ZDW with AA, n = 50). Furthermore, we found that the expression levels of tnfα and mpo were decreased either in pretreatment or cotreatment groups. In conclusion, our findings revealed that AA-induced nephrotoxicities can be attenuated by ZDW. Therefore, we believe that zebrafish represent an efficient model for screening AA-protective Chinese medicine.