PUBLICATION
High-throughput preparation of radioprotective polymers via Hantzsch's reaction for in vivo X-ray damage determination
- Authors
- Liu, G., Zeng, Y., Lv, T., Mao, T., Wei, Y., Jia, S., Gou, Y., Tao, L.
- ID
- ZDB-PUB-201208-38
- Date
- 2020
- Source
- Nature communications 11: 6214 (Journal)
- Registered Authors
- Jia, Shunji
- Keywords
- none
- MeSH Terms
-
- Amifostine/pharmacology
- Animals
- Cell Line
- Cell Survival/drug effects
- Cell Survival/radiation effects
- Chemistry Techniques, Synthetic/methods*
- Comet Assay
- DNA Damage/drug effects
- DNA Damage/radiation effects
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/radiation effects*
- Fibroblasts/cytology
- Fibroblasts/drug effects
- Fibroblasts/radiation effects*
- Mice
- Models, Chemical
- Molecular Structure
- Polymers/chemical synthesis*
- Polymers/chemistry
- Polymers/pharmacology
- Radiation-Protective Agents/chemical synthesis*
- Radiation-Protective Agents/chemistry
- Radiation-Protective Agents/pharmacology
- X-Rays*
- Zebrafish/embryology
- PubMed
- 33277480 Full text @ Nat. Commun.
Citation
Liu, G., Zeng, Y., Lv, T., Mao, T., Wei, Y., Jia, S., Gou, Y., Tao, L. (2020) High-throughput preparation of radioprotective polymers via Hantzsch's reaction for in vivo X-ray damage determination. Nature communications. 11:6214.
Abstract
Radioprotectors for acute injuries caused by large doses of ionizing radiation are vital to national security, public health and future development of humankind. Here, we develop a strategy to explore safe and efficient radioprotectors by combining Hantzsch's reaction, high-throughput methods and polymer chemistry. A water-soluble polymer with low-cytotoxicity and an excellent anti-radiation capability has been achieved. In in vivo experiments, this polymer is even better than amifostine, which is the only approved radioprotector for clinical applications, in effectively protecting zebrafish embryos from fatally large doses of ionizing radiation (80 Gy X-ray). A mechanistic study also reveals that the radioprotective ability of this polymer originates from its ability to efficiently prevent DNA damage due to high doses of radiation. This is an initial attempt to explore polymer radioprotectors via a multi-component reaction. It allows exploiting functional polymers and provides the underlying insights to guide the design of radioprotective polymers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping