PUBLICATION
Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo
- Authors
- Dawes, M.L., Soeller, C., Scholpp, S.
- ID
- ZDB-PUB-201020-49
- Date
- 2020
- Source
- Histochemistry and cell biology 154(5): 507-519 (Review)
- Registered Authors
- Scholpp, Steffen
- Keywords
- Fluorescent correlation spectroscopy (FCS), Microscopy, Protein–protein interactions, Zebrafish
- MeSH Terms
-
- Animals
- Molecular Dynamics Simulation
- Proteins/chemistry
- Proteins/metabolism*
- Spectrometry, Fluorescence
- Zebrafish/embryology*
- PubMed
- 33067656 Full text @ Histochem. Cell Biol.
Citation
Dawes, M.L., Soeller, C., Scholpp, S. (2020) Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo. Histochemistry and cell biology. 154(5):507-519.
Abstract
Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping