PUBLICATION
Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish
- Authors
- Wen, H., Eckenstein, K., Weihrauch, V., Stigloher, C., Brehm, P.
- ID
- ZDB-PUB-201007-4
- Date
- 2020
- Source
- Proceedings of the National Academy of Sciences of the United States of America 117(42): 26429-26437 (Journal)
- Registered Authors
- Stigloher, Christian
- Keywords
- N-type calcium channels, P/Q-type calcium channels, conotoxins, neuromuscular, synaptic
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Calcium Channels/metabolism*
- Calcium Channels/physiology*
- Calcium Channels, N-Type/metabolism
- Calcium Channels, P-Type/metabolism
- Calcium Channels, Q-Type/metabolism
- Escape Reaction/physiology
- Motor Neurons/metabolism*
- Motor Neurons/physiology
- Neuromuscular Junction/metabolism
- Presynaptic Terminals/physiology
- Swimming/physiology
- Synapses/metabolism
- Zebrafish/metabolism
- PubMed
- 33020266 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Wen, H., Eckenstein, K., Weihrauch, V., Stigloher, C., Brehm, P. (2020) Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 117(42):26429-26437.
Abstract
The escape response and rhythmic swimming in zebrafish are distinct behaviors mediated by two functionally distinct motoneuron (Mn) types. The primary (1°Mn) type depresses and has a large quantal content (Qc) and a high release probability (Pr). Conversely, the secondary (2°Mn) type facilitates and has low and variable Qc and Pr. This functional duality matches well the distinct associated behaviors, with the 1°Mn providing the strong, singular C bend initiating escape and the 2°Mn conferring weaker, rhythmic contractions. Contributing to these functional distinctions is our identification of P/Q-type calcium channels mediating transmitter release in 1°Mns and N-type channels in 2°Mns. Remarkably, despite these functional and behavioral distinctions, all ∼15 individual synapses on each muscle cell are shared by a 1°Mn bouton and at least one 2°Mn bouton. This blueprint of synaptic sharing provides an efficient way of controlling two different behaviors at the level of a single postsynaptic cell.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping