PUBLICATION
Individual neuronal subtypes control initial myelin sheath growth and stabilization
- Authors
- Nelson, H.N., Treichel, A.J., Eggum, E.N., Martell, M.R., Kaiser, A.J., Trudel, A.G., Gronseth, J.R., Maas, S.T., Bergen, S., Hines, J.H.
- ID
- ZDB-PUB-201002-199
- Date
- 2020
- Source
- Neural Development 15: 12 (Journal)
- Registered Authors
- Hines, Jacob H.
- Keywords
- Glia, Myelin sheath, Myelination, Oligodendrocyte
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/physiology*
- Embryo, Nonmammalian
- Genes, Reporter
- Larva
- Myelin Sheath/physiology*
- Oligodendroglia/physiology*
- Spinal Cord/physiology*
- Zebrafish
- PubMed
- 32988384 Full text @ Neural Dev.
Citation
Nelson, H.N., Treichel, A.J., Eggum, E.N., Martell, M.R., Kaiser, A.J., Trudel, A.G., Gronseth, J.R., Maas, S.T., Bergen, S., Hines, J.H. (2020) Individual neuronal subtypes control initial myelin sheath growth and stabilization. Neural Development. 15:12.
Abstract
Background In the developing central nervous system, pre-myelinating oligodendrocytes sample candidate nerve axons by extending and retracting process extensions. Some contacts stabilize, leading to the initiation of axon wrapping, nascent myelin sheath formation, concentric wrapping and sheath elongation, and sheath stabilization or pruning by oligodendrocytes. Although axonal signals influence the overall process of myelination, the precise oligodendrocyte behaviors that require signaling from axons are not completely understood. In this study, we investigated whether oligodendrocyte behaviors during the early events of myelination are mediated by an oligodendrocyte-intrinsic myelination program or are over-ridden by axonal factors.
Methods To address this, we utilized in vivo time-lapse imaging in embryonic and larval zebrafish spinal cord during the initial hours and days of axon wrapping and myelination. Transgenic reporter lines marked individual axon subtypes or oligodendrocyte membranes.
Results In the larval zebrafish spinal cord, individual axon subtypes supported distinct nascent sheath growth rates and stabilization frequencies. Oligodendrocytes ensheathed individual axon subtypes at different rates during a two-day period after initial axon wrapping. When descending reticulospinal axons were ablated, local spinal axons supported a constant ensheathment rate despite the increased ratio of oligodendrocytes to target axons.
Conclusion We conclude that properties of individual axon subtypes instruct oligodendrocyte behaviors during initial stages of myelination by differentially controlling nascent sheath growth and stabilization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping