PUBLICATION

The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish

Authors
Converse, A., Thomas, P.
ID
ZDB-PUB-201002-162
Date
2020
Source
Scientific Reports   10: 15673 (Journal)
Registered Authors
Koch Converse, Aubrey, Thomas, Peter
Keywords
none
MeSH Terms
  • Animals
  • Calcium/metabolism
  • Gene Expression Regulation, Developmental
  • Larva/growth & development
  • Oocytes/physiology
  • Ovum/physiology*
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Zinc/metabolism*
PubMed
32973303 Full text @ Sci. Rep.
Citation
Converse, A., Thomas, P. (2020) The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish. Scientific Reports. 10:15673.
Abstract
The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To examine the role of ZIP9 in ovarian physiology, we generated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system. zip9-/- females showed significant reductions in fecundity, embryo viability, and growth of their offspring compared to wildtype (WT) fish. Furthermore, a high proportion of zip9-/- eggs failed to undergo normal chorion elevation during activation. In WT eggs, zinc was detected in cortically-localized vesicles which underwent exocytosis upon activation. zip9-/- eggs showed abnormal cortical vesicle development and had a significantly depressed activation-induced zinc release compared to WT eggs. Moreover, pharmacologically sustained elevation of zinc in WT eggs prior to activation resulted in abnormal chorion elevation similar to that observed in zip9-/- eggs. These results indicate that ZIP9 is essential for proper zinc modulation during zebrafish egg activation and presents the first evidence of zinc modulation during egg activation in a non-mammalian species.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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