PUBLICATION
Live-imaging of astrocyte morphogenesis and function in zebrafish neural circuits
- Authors
- Chen, J., Poskanzer, K.E., Freeman, M.R., Monk, K.R.
- ID
- ZDB-PUB-200909-3
- Date
- 2020
- Source
- Nature Neuroscience 23(10): 1297-1306 (Other)
- Registered Authors
- Monk, Kelly
- Keywords
- none
- MeSH Terms
-
- Animals
- Astrocytes/physiology*
- Brain/growth & development*
- Calcium Signaling
- Ependymoglial Cells/physiology*
- Morphogenesis*
- Neural Pathways/physiology
- Neurons/physiology*
- Receptor, Fibroblast Growth Factor, Type 3/physiology
- Receptor, Fibroblast Growth Factor, Type 4/physiology
- Spinal Cord/growth & development*
- Synapses/physiology
- Zebrafish/growth & development*
- Zebrafish Proteins/physiology
- PubMed
- 32895565 Full text @ Nat. Neurosci.
Citation
Chen, J., Poskanzer, K.E., Freeman, M.R., Monk, K.R. (2020) Live-imaging of astrocyte morphogenesis and function in zebrafish neural circuits. Nature Neuroscience. 23(10):1297-1306.
Abstract
How astrocytes grow and integrate into neural circuits remains poorly defined. Zebrafish are well suited for such investigations, but bona fide astrocytes have not been described in this system. Here we characterize a zebrafish cell type that is remarkably similar to mammalian astrocytes that derive from radial glial cells and elaborate processes to establish their territories at early larval stages. Zebrafish astrocytes associate closely with synapses, tile with one another and express markers, including Glast and glutamine synthetase. Once integrated into circuits, they exhibit whole-cell and microdomain Ca2+ transients, which are sensitive to norepinephrine. Finally, using a cell-specific CRISPR-Cas9 approach, we demonstrate that fgfr3 and fgfr4 are required for vertebrate astrocyte morphogenesis. This work provides the first visualization of astrocyte morphogenesis from stem cell to post-mitotic astrocyte in vivo, identifies a role for Fgf receptors in vertebrate astrocytes and establishes zebrafish as a valuable new model system to study astrocyte biology in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping