PUBLICATION

Microplastic-associated trophic transfer of benzo(k)fluoranthene in a limnic food web: Effects in two freshwater invertebrates (Daphnia magna, Chironomus riparius) and zebrafish (Danio rerio)

Authors
Hanslik, L., Sommer, C., Huppertsberg, S., Dittmar, S., Knepper, T.P., Braunbeck, T.
ID
ZDB-PUB-200810-33
Date
2020
Source
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   237: 108849 (Journal)
Registered Authors
Braunbeck, Thomas
Keywords
CYP1A induction, GC–MS analysis of PAHs, Invertebrates, Microplastics, Trophic transfer, Zebrafish
MeSH Terms
  • Animals
  • Cytochrome P-450 CYP1A1/metabolism
  • Daphnia/growth & development*
  • Fluorenes/toxicity*
  • Food Chain*
  • Intestines/drug effects
  • Liver/drug effects
  • Liver/metabolism
  • Microplastics/toxicity*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/growth & development*
PubMed
32768657 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Hanslik, L., Sommer, C., Huppertsberg, S., Dittmar, S., Knepper, T.P., Braunbeck, T. (2020) Microplastic-associated trophic transfer of benzo(k)fluoranthene in a limnic food web: Effects in two freshwater invertebrates (Daphnia magna, Chironomus riparius) and zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 237:108849.
Abstract
The continuously growing plastic production and incomplete recycling processes open manifold entry routes for microplastic particles (MPs) into the environment. Since knowledge on trophic transfer of contaminants sorbed to MPs is still insufficient for freshwater systems, the transfer of the model pollutant benzo(k)fluoranthene (BkF) sorbed to polymethyl methacrylate (PMMA) particles in a limnic food web was investigated: Two freshwater invertebrates (Daphnia magna and Chironomus riparius larvae) were selected and either left untreated, exposed to pristine PMMA, PMMA-associated BkF, or exposed to dissolved BkF (BkFaq). As second-level consumers, zebrafish (Danio rerio) were fed twice daily with pre-treated invertebrates over two days. Induction of hepatic cytochrome P450 by BkF was determined as 7-ethoxy-O-resorufin deethylase (EROD) activity. Both invertebrate species readily ingested PMMA particles, tracked via fluorescence microscopy and accumulated BkFaq, measured via GC-MS. Fluorescence signals in gastrointestinal tracts of zebrafish were quantified with confocal laser scanning microscopy (CLSM). The fluorescence signal in gastrointestinal tracts of zebrafish was not altered, whereas, EROD activity was significantly induced when zebrafish were fed with Chironomus riparius, pre-exposed to BkFaq. Trophic exposure scenarios with BkF sorbed to PMMA did not result in any alterations of investigated endpoints in both invertebrate species and zebrafish compared to controls. Given that BkF amounts were in the low ng-range, as detected by GC-MS, the transport of MP-sorbed BkF to zebrafish was less effective than direct exposure to waterborne BkFaq, and the potential threat of trophic transfer of substances such as BkF in limnic food webs may have been overestimated.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.