PUBLICATION

Loss of Caveolin-1 and caveolae leads to increased cardiac cell stiffness and functional decline of the adult zebrafish heart

Authors
Grivas, D., González-Rajal, Á., Guerrero Rodríguez, C., Garcia, R., de la Pompa, J.L.
ID
ZDB-PUB-200801-5
Date
2020
Source
Scientific Reports   10: 12816 (Journal)
Registered Authors
de la Pompa, José Luis, Grivas, Dimitrios
Keywords
none
MeSH Terms
  • Animals
  • Cardiovascular Physiological Phenomena/genetics*
  • Caveolae*
  • Caveolin 1/genetics*
  • Caveolin 1/physiology*
  • Elasticity*
  • Gene Deletion*
  • Myocytes, Cardiac/pathology*
  • Myocytes, Cardiac/physiology*
  • Signal Transduction/physiology
  • Zebrafish*
PubMed
32733088 Full text @ Sci. Rep.
Abstract
Caveolin-1 is the main structural protein of caveolae, small membrane invaginations involved in signal transduction and mechanoprotection. Here, we generated cav1-KO zebrafish lacking Cav1 and caveolae, and investigated the impact of this loss on adult heart function and response to cryoinjury. We found that cardiac function was impaired in adult cav1-KO fish, which showed a significantly decreased ejection fraction and heart rate. Using atomic force microscopy, we detected an increase in the stiffness of epicardial cells and cells of the cortical zone lacking Cav1/caveolae. This loss of cardiac elasticity might explain the decreased cardiac contraction and function. Surprisingly, cav1-KO mutants were able to regenerate their heart after a cryoinjury but showed a transient decrease in cardiomyocyte proliferation.
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Human Disease / Model
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