PUBLICATION

Yolk sac-derived Pdcd11-positive cells modulate zebrafish microglia differentiation through the NF-κB-Tgfβ1 pathway

Authors
Yang, R., Zhan, M., Guo, M., Yuan, H., Wang, Y., Zhang, Y., Zhang, W., Chen, S., de The, H., Chen, Z., Zhou, J., Zhu, J.
ID
ZDB-PUB-200728-20
Date
2020
Source
Cell death and differentiation   28(1): 170-183 (Journal)
Registered Authors
Zhang, Wenqing, Zhang, Yiyue
Keywords
none
MeSH Terms
  • Animals
  • Brain/metabolism
  • Cell Differentiation/physiology*
  • Cytokines/metabolism
  • Inflammation/metabolism
  • Macrophages/metabolism
  • Microglia/metabolism*
  • NF-kappa B/metabolism*
  • Neurogenesis
  • Signal Transduction
  • Transforming Growth Factor beta1/metabolism*
  • Yolk Sac/metabolism
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed
32709934 Full text @ Cell Death Differ.
Abstract
Microglia are the primary immune cells in the central nervous system, which plays a vital role in neuron development and neurodegenerative diseases. Microglial precursors in peripheral hematopoietic tissues colonize the central nervous system during early embryogenesis. However, how intrinsic and extrinsic signals integrate to regulate microglia's differentiation remains undefined. In this study, we identified the cerebral white matter hyperintensities susceptibility gene, programmed cell death protein 11 (PDCD11), as an essential factor regulating microglia differentiation. In zebrafish, pdcd11 deficiency prevents the differentiation of the precursors to mature brain microglia. Although, the inflammatory featured macrophage brain colonization is augmented. At 22 h post fertilization, the Pdcd11-positive cells on the yolk sac are distinct from macrophages and neutrophils. Mechanistically, PDCD11 exerts its physiological role by differentially regulating the functions of nuclear factor-kappa B family members, P65 and c-Rel, suppressing P65-mediated expression of inflammatory cytokines, such as tnfα, and enhancing the c-Rel-dependent appearance of tgfβ1. The present study provides novel insights in understanding microglia differentiation during zebrafish development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping