PUBLICATION

A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity

Authors
Pende, M., Vadiwala, K., Schmidbaur, H., Stockinger, A.W., Murawala, P., Saghafi, S., Dekens, M.P.S., Becker, K., Revilla-I-Domingo, R., Papadopoulos, S.C., Zurl, M., Pasierbek, P., Simakov, O., Tanaka, E.M., Raible, F., Dodt, H.U.
ID
ZDB-PUB-200612-5
Date
2020
Source
Science advances   6: eaba0365 (Journal)
Registered Authors
Dekens, Marcus P.S., Raible, Florian
Keywords
none
MeSH Terms
none
PubMed
32523996 Full text @ Sci Adv
Abstract
Tissue clearing combined with deep imaging has emerged as a powerful alternative to classical histological techniques. Whereas current techniques have been optimized for imaging selected nonpigmented organs such as the mammalian brain, natural pigmentation remains challenging for most other biological specimens of larger volume. We have developed a fast DEpigmEntation-Plus-Clearing method (DEEP-Clear) that is easily incorporated in existing workflows and combines whole system labeling with a spectrum of detection techniques, ranging from immunohistochemistry to RNA in situ hybridization, labeling of proliferative cells (EdU labeling) and visualization of transgenic markers. With light-sheet imaging of whole animals and detailed confocal studies on pigmented organs, we provide unprecedented insight into eyes, whole nervous systems, and subcellular structures in animal models ranging from worms and squids to axolotls and zebrafish. DEEP-Clear thus paves the way for the exploration of species-rich clades and developmental stages that are largely inaccessible by regular imaging approaches.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping