PUBLICATION
Acute Regulation of Habituation Learning via Posttranslational Palmitoylation
- Authors
- Nelson, J.C., Witze, E., Ma, Z., Ciocco, F., Frerotte, A., Randlett, O., Foskett, J.K., Granato, M.
- ID
- ZDB-PUB-200606-33
- Date
- 2020
- Source
- Current biology : CB 30(14): 2729-2738.e4 (Journal)
- Registered Authors
- Granato, Michael, Randlett, Owen
- Keywords
- Hip14, Kv1.1, Mauthner, Zdhhc17, axon cap, behavior, genetic screen, habituation learning, startle, zebrafish
- MeSH Terms
-
- Presynaptic Terminals/metabolism
- Acyltransferases/physiology*
- Protein Processing, Post-Translational/genetics*
- Protein Processing, Post-Translational/physiology*
- Nerve Tissue Proteins/physiology*
- Animals
- Zebrafish/genetics*
- Zebrafish/physiology*
- Adaptor Proteins, Signal Transducing/physiology*
- Shaker Superfamily of Potassium Channels/metabolism
- Shaker Superfamily of Potassium Channels/physiology*
- Habituation, Psychophysiologic/genetics*
- Learning/physiology*
- Lipoylation/genetics*
- Lipoylation/physiology*
- PubMed
- 32502414 Full text @ Curr. Biol.
Citation
Nelson, J.C., Witze, E., Ma, Z., Ciocco, F., Frerotte, A., Randlett, O., Foskett, J.K., Granato, M. (2020) Acute Regulation of Habituation Learning via Posttranslational Palmitoylation. Current biology : CB. 30(14):2729-2738.e4.
Abstract
Habituation is an adaptive learning process that enables animals to adjust innate behaviors to changes in their environment. Despite its well-documented implications for a wide diversity of behaviors, the molecular and cellular basis of habituation learning is not well understood. Using whole-genome sequencing of zebrafish mutants isolated in an unbiased genetic screen, we identified the palmitoyltransferase Huntingtin interacting protein 14 (Hip14) as a critical regulator of habituation learning. We demonstrate that Hip14 regulates depression of sensory inputs onto an identified hindbrain neuron and provide evidence that Hip14 palmitoylates the Shaker-like K+ voltage-gated channel subunit (Kv1.1), thereby regulating Kv1.1 subcellular localization. Furthermore, we show that, like for Hip14, loss of Kv1.1 leads to habituation deficits and that Hip14 is dispensable in development and instead acts acutely to promote habituation. Combined, these results uncover a previously unappreciated role for acute posttranslational palmitoylation at defined circuit components to regulate learning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping