PUBLICATION

Acute Regulation of Habituation Learning via Posttranslational Palmitoylation

Authors
Nelson, J.C., Witze, E., Ma, Z., Ciocco, F., Frerotte, A., Randlett, O., Foskett, J.K., Granato, M.
ID
ZDB-PUB-200606-33
Date
2020
Source
Current biology : CB   30(14): 2729-2738.e4 (Journal)
Registered Authors
Granato, Michael, Randlett, Owen
Keywords
Hip14, Kv1.1, Mauthner, Zdhhc17, axon cap, behavior, genetic screen, habituation learning, startle, zebrafish
MeSH Terms
  • Protein Processing, Post-Translational/genetics*
  • Protein Processing, Post-Translational/physiology*
  • Habituation, Psychophysiologic/genetics*
  • Lipoylation/genetics*
  • Lipoylation/physiology*
  • Animals
  • Zebrafish/genetics*
  • Zebrafish/physiology*
  • Presynaptic Terminals/metabolism
  • Learning/physiology*
  • Adaptor Proteins, Signal Transducing/physiology*
  • Shaker Superfamily of Potassium Channels/metabolism
  • Shaker Superfamily of Potassium Channels/physiology*
  • Acyltransferases/physiology*
  • Nerve Tissue Proteins/physiology*
(all 15)
PubMed
32502414 Full text @ Curr. Biol.
Abstract
Habituation is an adaptive learning process that enables animals to adjust innate behaviors to changes in their environment. Despite its well-documented implications for a wide diversity of behaviors, the molecular and cellular basis of habituation learning is not well understood. Using whole-genome sequencing of zebrafish mutants isolated in an unbiased genetic screen, we identified the palmitoyltransferase Huntingtin interacting protein 14 (Hip14) as a critical regulator of habituation learning. We demonstrate that Hip14 regulates depression of sensory inputs onto an identified hindbrain neuron and provide evidence that Hip14 palmitoylates the Shaker-like K+ voltage-gated channel subunit (Kv1.1), thereby regulating Kv1.1 subcellular localization. Furthermore, we show that, like for Hip14, loss of Kv1.1 leads to habituation deficits and that Hip14 is dispensable in development and instead acts acutely to promote habituation. Combined, these results uncover a previously unappreciated role for acute posttranslational palmitoylation at defined circuit components to regulate learning.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
kcna1ankhspgffdmc130AEt; p201Tgstandard conditionsDay 5IHC
kcna1azdhhc17p174/p174; nkhspgffdmc130AEt; p201Tgstandard conditionsDay 5IHC
kcna1azdhhc17p408/p408; nkhspgffdmc130AEt; p201Tgstandard conditionsDay 5IHC
1 - 3 of 3
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Phenotype
Fish Conditions Stage Phenotype Figure
kcna1ap181/+acoustic radiationDay 5
kcna1ap181/+; kcna1ap410/+acoustic radiationDay 5
kcna1ap181/p181acoustic radiationDay 5
kcna1ap181/p181acoustic radiationDay 5
kcna1ap410/+acoustic radiationDay 5
kcna1ap410/p410acoustic radiationDay 5
zdhhc17p174/+acoustic radiationDay 5
zdhhc17p174/+acoustic radiationDay 5
zdhhc17p174/+; zdhhc17p408/+acoustic radiationDay 5
zdhhc17p174/+; zdhhc17p408/+acoustic radiationDay 5
1 - 10 of 33
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a150TgTransgenic Insertion
    a151TgTransgenic Insertion
      nkhspGFF62ATgTransgenic Insertion
        nkhspgffdmc130AEtTransgenic Insertion
          p170
            		Point Mutation
            p174
              		Point Mutation
              p181
                		Point Mutation
                p186TgTransgenic Insertion
                  p201TgTransgenic Insertion
                    p408
                      		Small Deletion
                      1 - 10 of 12
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Fish
                      kcna1ap181/p181
                      kcna1ap181/+
                      kcna1ap181/+; kcna1ap410/+
                      kcna1ap410/p410
                      kcna1ap410/+
                      nkhspgffdmc130AEt; p201Tg
                      p409Tg
                      zdhhc17p174/p174
                      zdhhc17p174/p174; nkhspgffdmc130AEt; p201Tg
                      zdhhc17p174/p174; p409Tg
                      1 - 10 of 16
                      Show
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CitrineEFGCitrine
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GAL4FFEFGGAL4FF
                      GCaMPEFGGCaMP
                      mKateEFGmKate
                      1 - 6 of 6
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                      Mapping
                      No data available
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                      Citation
                      Nelson, J.C., Witze, E., Ma, Z., Ciocco, F., Frerotte, A., Randlett, O., Foskett, J.K., Granato, M. (2020) Acute Regulation of Habituation Learning via Posttranslational Palmitoylation. Current biology : CB. 30(14):2729-2738.e4.