PUBLICATION

Effects of BPA on zebrafish gonads: Focus on the endocannabinoid system

Authors
Forner-Piquer, I., Beato, S., Piscitelli, F., Santangeli, S., Di Marzo, V., Habibi, H.R., Maradonna, F., Carnevali, O.
ID
ZDB-PUB-200518-15
Date
2020
Source
Environmental pollution (Barking, Essex : 1987)   264: 114710 (Journal)
Registered Authors
Carnevali, Oliana
Keywords
Danio rerio, Endocannabinoid, Endocrine disruptors, Reproduction, Vitellogenin
MeSH Terms
  • Animals
  • Benzhydryl Compounds/pharmacology
  • Endocannabinoids
  • Endocrine Disruptors/pharmacology*
  • Female
  • Gonads/drug effects
  • Male
  • Phenols
  • Reproduction
  • Vitellogenins
  • Water Pollutants, Chemical/pharmacology*
  • Zebrafish
PubMed
32417572 Full text @ Environ. Pollut.
Abstract
Bisphenol A (BPA), a monomer used for polycarbonate manufacture, has been widely reported as an endocrine-disrupting chemical (EDC). Among other alterations, BPA induces reproductive dysfunctionalities. Changes in the endocannabinoid system (ECS) have been recently shown to be associated with reproductive disorders. The ECS is a lipid-based signaling system (cannabinoid receptors, endocannabinoids and enzymatic machinery) involved in several physiological functions. The main goal of the present study was to assess the effects of two environmental concentrations of BPA (10 and 20 μg/L) on the ECS in 1-year old zebrafish gonads. In males, BPA increased the gonadosomatic index (GSI) and altered testicular levels of endocannabinoids as well as reduced the testicular area occupied by spermatogonia. In male liver, exposure to 20 μg/L BPA significantly increased vitellogenin (vtg) transcript levels. In female zebrafish, BPA altered ovarian endocannabinoid levels, elevated hepatic vtg mRNA levels as well as increased the percentage of vitellogenic oocytes in the ovaries. In conclusion, exposure to two environmentally relevant concentrations of BPA altered the ECS and consequently, gonadal function in both male and female zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping