PUBLICATION

Identification of type I IFNs and their receptors in a cyprinid fish, the topmouth culter Culter alburnus

Authors
Li, B., Chen, S.N., Ren, L., Wang, S., Liu, L., Liu, Y., Liu, S., Nie, P.
ID
ZDB-PUB-200511-6
Date
2020
Source
Fish & shellfish immunology   102: 326-335 (Journal)
Registered Authors
Nie, Pin
Keywords
CRFB, Fish, Topmouth culter, Type I IFN
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cyprinidae/genetics*
  • Cyprinidae/immunology*
  • Fish Proteins/chemistry
  • Fish Proteins/genetics
  • Fish Proteins/immunology
  • Gene Expression Profiling/veterinary
  • Gene Expression Regulation/immunology*
  • Immunity/genetics*
  • Interferon Type I/chemistry
  • Interferon Type I/genetics*
  • Interferon Type I/immunology
  • Phylogeny
  • Poly I-C/pharmacology
  • Receptors, Interferon/chemistry
  • Receptors, Interferon/genetics*
  • Receptors, Interferon/immunology
  • Sequence Alignment/veterinary
PubMed
32387477 Full text @ Fish Shellfish Immunol.
Abstract
In fish, type I IFNs are classified into three groups, i.e. group one, group two and group three, and further separated into seven subgroups based on the number of conserved cysteines and phylogenetic relationships. In the present study, four type I IFNs, named as IFNϕ1, IFNϕ2, IFNϕ3, IFNϕ4, as reported in zebrafish, were identified in a cyprinid, the topmouth culter, Culter alburnus, a species introduced recently into China's aquaculture. These IFNs may be classified as IFNa, IFNc, IFNc and IFNd in a recent nomenclature, with IFNa and IFNd having two cysteines in group one, and IFNc four cysteines in group two. These IFNs, together with their possible receptors, IFNϕ1, IFNϕ2, IFNϕ3, IFNϕ4, and CRFB1, CRFB2 and CRFB5 have an open reading frame (ORF) of 540, 552, 567, 516 bp, and 1572, 1392, 1125 bp, respectively. These IFNs have high amino acid sequence identities, being 91.1-93.6% and 66.9-77.3%, with those in grass carp and zebrafish, respectively, and are expressed constitutively in organs/tissues examined in the fish. The expression of these IFNs can be further induced following poly (I:C) stimulation. However, the possible function of these IFNs and their signalling pathway are of interest for further research.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes