PUBLICATION
Genome-wide Survey of Ribosome Collision
- Authors
- Han, P., Shichino, Y., Schneider-Poetsch, T., Mito, M., Hashimoto, S., Udagawa, T., Kohno, K., Yoshida, M., Mishima, Y., Inada, T., Iwasaki, S.
- ID
- ZDB-PUB-200507-11
- Date
- 2020
- Source
- Cell Reports 31: 107610 (Journal)
- Registered Authors
- Mishima, Yuichiro
- Keywords
- XBP, disome, disome profiling, eIF5A, ribosome, ribosome collision, ribosome profiling, ribosome-associated quality control (RQC), translation, translation elongation
- Datasets
- GEO:GSE133392
- MeSH Terms
-
- 3' Untranslated Regions/genetics
- Animals
- Codon, Terminator/genetics*
- Codon, Terminator/metabolism
- Humans
- Peptide Chain Elongation, Translational/genetics
- Protein Biosynthesis/genetics*
- RNA, Messenger/metabolism
- Ribosomes/genetics*
- Ribosomes/metabolism*
- Saccharomyces cerevisiae Proteins/metabolism
- Zebrafish
- PubMed
- 32375038 Full text @ Cell Rep.
Citation
Han, P., Shichino, Y., Schneider-Poetsch, T., Mito, M., Hashimoto, S., Udagawa, T., Kohno, K., Yoshida, M., Mishima, Y., Inada, T., Iwasaki, S. (2020) Genome-wide Survey of Ribosome Collision. Cell Reports. 31:107610.
Abstract
Ribosome movement is not always smooth and is rather often impeded. For ribosome pauses, fundamental issues remain to be addressed, including where ribosomes pause on mRNAs, what kind of RNA/amino acid sequence causes this pause, and the physiological significance of this attenuation of protein synthesis. Here, we survey the positions of ribosome collisions caused by ribosome pauses in humans and zebrafish using modified ribosome profiling. Collided ribosomes, i.e., disomes, emerge at various sites: Pro-Pro/Gly/Asp motifs; Arg-X-Lys motifs; stop codons; and 3' untranslated regions. The electrostatic interaction between the charged nascent chain and the ribosome exit tunnel determines the eIF5A-mediated disome rescue at the Pro-Pro sites. In particular, XBP1u, a precursor of endoplasmic reticulum (ER)-stress-responsive transcription factor, shows striking queues of collided ribosomes and thus acts as a degradation substrate by ribosome-associated quality control. Our results provide insight into the causes and consequences of ribosome pause by dissecting collided ribosomes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping