PUBLICATION
Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale
- Authors
- Kobayashi-Sun, J., Yamamori, S., Kondo, M., Kuroda, J., Ikegame, M., Suzuki, N., Kitamura, K.I., Hattori, A., Yamaguchi, M., Kobayashi, I.
- ID
- ZDB-PUB-200426-11
- Date
- 2020
- Source
- Communications biology 3: 190 (Journal)
- Registered Authors
- Kobayashi, Isao, Yamaguchi, Masahiro
- Keywords
- none
- Datasets
- GEO:GSE134330
- MeSH Terms
-
- Animal Scales/metabolism*
- Animals
- Animals, Genetically Modified
- Cell Differentiation*
- Cells, Cultured
- Extracellular Vesicles/genetics
- Extracellular Vesicles/metabolism
- Extracellular Vesicles/transplantation*
- Fracture Healing*
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Models, Animal
- Osteoblasts/metabolism
- Osteoblasts/transplantation*
- Osteoclasts/metabolism*
- Osteogenesis*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 32327701 Full text @ Commun Biol
Citation
Kobayashi-Sun, J., Yamamori, S., Kondo, M., Kuroda, J., Ikegame, M., Suzuki, N., Kitamura, K.I., Hattori, A., Yamaguchi, M., Kobayashi, I. (2020) Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale. Communications biology. 3:190.
Abstract
Differentiation of osteoclasts (OCs) from hematopoietic cells requires cellular interaction with osteoblasts (OBs). Due to the difficulty of live-imaging in the bone, however, the cellular and molecular mechanisms underlying intercellular communication involved in OC differentiation are still elusive. Here, we develop a fracture healing model using the scale of trap:GFP; osterix:mCherry transgenic zebrafish to visualize the interaction between OCs and OBs. Transplantation assays followed by flow cytometric analysis reveal that most trap:GFPhigh OCs in the fractured scale are detected in the osterix:mCherry+ fraction because of uptake of OB-derived extracellular vesicles (EVs). In vivo live-imaging shows that immature OCs actively interact with osterix:mCherry+ OBs and engulf EVs prior to convergence at the fracture site. In vitro cell culture assays show that OB-derived EVs promote OC differentiation via Rankl signaling. Collectively, these data suggest that EV-mediated intercellular communication with OBs plays an important role in the differentiation of OCs in bone tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping