PUBLICATION
Molecular and functional characterization of tilapia DDX41 in IFN regulation
- Authors
- Gan, Z., Cheng, J., Hou, J., Xia, H., Chen, W., Xia, L., Nie, P., Lu, Y.
- ID
- ZDB-PUB-200225-10
- Date
- 2020
- Source
- Fish & shellfish immunology 99: 386-391 (Journal)
- Registered Authors
- Nie, Pin
- Keywords
- Cytosolic DNA sensors, DDX41, Oreochromis niloticus, Promoter activity, Type I interferon
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- DEAD-box RNA Helicases/genetics
- DEAD-box RNA Helicases/metabolism*
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Gene Expression Regulation, Enzymologic/immunology*
- Interferons/genetics
- Interferons/metabolism*
- Phylogeny
- Tilapia/metabolism*
- PubMed
- 32081808 Full text @ Fish Shellfish Immunol.
Citation
Gan, Z., Cheng, J., Hou, J., Xia, H., Chen, W., Xia, L., Nie, P., Lu, Y. (2020) Molecular and functional characterization of tilapia DDX41 in IFN regulation. Fish & shellfish immunology. 99:386-391.
Abstract
DEAD-box helicase 41 (DDX41) is a key cytosolic DNA sensor playing critical roles in the regulation of type I IFN responses, and their functions have been well-characterized in mammals. However, little information is available regarding the function of fish DDX41. In this study, a DDX41 gene, named On-DDX41, was identified in Nile tilapia, Oreochromis niloticus. The predicted protein of On-DDX41 contains several structural features known in DDX41, including conserved DEADc and HELICc domains, and a conserved sequence "Asp-Glu-Ala-Asp (D-E-A-D)". On-DDX41 gene was constitutively expressed in all tissues examined, with the highest expression level observed in liver and muscle, and was inducible after poly(I:C) stimulation. Moreover, the overexpression of On-DDX41 can elicit a strong activation of both zebrafish IFN1 and IFN3 promoter in fish cells treated with poly(dA:dT). The present study thus contributes to a better understanding of the functional properties of DDX41 in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping