PUBLICATION
Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
- Authors
- Arora, P., Dongre, S., Raman, R., Sonawane, M.
- ID
- ZDB-PUB-200123-5
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Sonawane, Mahendra
- Keywords
- E-cadherin, aPKC, cell biology, cell polarity, developmental biology, epidermis, zebrafish
- MeSH Terms
-
- Adherens Junctions/metabolism
- Animals
- Cadherins/analysis
- Cadherins/metabolism*
- Cell Polarity/physiology*
- Embryo, Nonmammalian
- Epidermal Cells/cytology*
- Epidermal Cells/metabolism
- Epidermis/embryology*
- Protein Kinase C/analysis
- Protein Kinase C/metabolism*
- Zebrafish
- PubMed
- 31967543 Full text @ Elife
Citation
Arora, P., Dongre, S., Raman, R., Sonawane, M. (2020) Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish. eLIFE. 9:.
Abstract
The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that both the periderm and the basal epidermis exhibit polarised distribution of adherens junctions in zebrafish. aPKC, an apical polarity regulator, maintains the robustness of polarisation of E-cadherin- an adherens junction component- in the periderm. E-cadherin in one layer controls the localisation of E-cadherin in the second layer in a layer non-autonomous manner. Importantly, E-cadherin controls the localisation and levels of Lgl, a basolateral polarity regulator, in a layer autonomous as well non-autonomous manner. Since periderm formation from the enveloping layer precedes the formation of the basal epidermis, our analyses suggest that peridermal polarity, initiated by aPKC, is transduced in a stepwise manner by E-cadherin to the basal layer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping