PUBLICATION
Patterns of spon1b:GFP expression during early zebrafish brain development
- Authors
- Agudelo-Dueñas, N., Forero-Shelton, M., Zhdanova, I.V., Akle, V.
- ID
- ZDB-PUB-200109-2
- Date
- 2020
- Source
- BMC research notes 13: 14 (Journal)
- Registered Authors
- Zhdanova, Irina
- Keywords
- F-spondin/spon1b, GFP, Habenula, Light sheet fluorescence microscopy (LSFM), Olfactory system, Pituitary/hypophysis, Zebrafish (Danio rerio)
- MeSH Terms
-
- Animals
- Brain/embryology*
- Brain/metabolism*
- Embryo, Nonmammalian/metabolism
- Extracellular Matrix Proteins/genetics*
- Extracellular Matrix Proteins/metabolism
- Fertilization
- Gene Expression Regulation, Developmental*
- Green Fluorescent Proteins/genetics*
- Green Fluorescent Proteins/metabolism
- Habenula/embryology
- Habenula/metabolism
- Zebrafish/embryology*
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 31910899 Full text @ BMC Res. Notes
Citation
Agudelo-Dueñas, N., Forero-Shelton, M., Zhdanova, I.V., Akle, V. (2020) Patterns of spon1b:GFP expression during early zebrafish brain development. BMC research notes. 13:14.
Abstract
Objective F-spondin is part of a group of evolutionarily conserved extracellular matrix proteins in vertebrates. It is highly expressed in the embryonic floor plate, and it can bind to the ECM and promote neuronal outgrowth. A characterization of F-spondin expression patterns in the adult zebrafish brain was previously reported by our group. However, given its importance during development, we aimed to obtain a detailed description of green fluorescent protein (GFP) expression driven by the spon1b promotor, in the developing zebrafish brain of the transgenic Tg(spon1b:GFP) line, using light sheet fluorescence microscopy (LSFM).
Results Images obtained in live embryos from 22 to 96 h post fertilization confirmed our earlier reports on the presence of spon1b:GFP expressing cells in the telencephalon and diencephalon (olfactory bulbs, habenula, optic tectum, nuclei of the medial longitudinal fasciculus), and revealed new spon1b:GFP populations in the pituitary anlage, dorso-rostral cluster, and ventro-rostral cluster. LSFM made it possible to follow the dynamics of cellular migration patterns during development.
Conclusions spon1b:GFP larval expression patterns starts in early development in specific neuronal structures of the developing brain associated with sensory-motor modulation. LSFM evaluation of the transgenic Tg(spon1b:GFP) line provides an effective approach to characterize GFP expression patterns in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping