PUBLICATION

Regeneration of the gill filaments and replacement of serotonergic neuroepithelial cells in adult zebrafish (Danio rerio)

Authors
Mierzwa, A.S., Nguyen, F., Xue, M., Jonz, M.G.
ID
ZDB-PUB-200104-9
Date
2019
Source
Respiratory Physiology & Neurobiology   274: 103366 (Journal)
Registered Authors
Jonz, Michael G.
Keywords
Gill, chemoreceptor, neuroepithelial cell, regeneration, serotonin, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Disease Models, Animal
  • Gills/blood supply
  • Gills/diagnostic imaging
  • Gills/innervation
  • Gills/physiology*
  • Immunohistochemistry
  • Microscopy, Confocal
  • Neuroepithelial Cells/physiology*
  • Proliferating Cell Nuclear Antigen
  • Regeneration/physiology*
  • Serotonergic Neurons/physiology*
  • Zebrafish/physiology*
  • Zebrafish Proteins
PubMed
31899351 Full text @ Respir. Physiol. Neurobiol.
Abstract
Respiratory epithelia and chemoreceptors of the gills and mammalian lung derive from the same embryonic structures. While the lung is limited to facultative regeneration, the regenerative capacity of the gill has not been adequately explored. We report regeneration of gill filaments and respiratory lamellae in adult zebrafish (Danio rerio). Gill filaments retained a constitutive population of mitotic cells identified by the proliferating cell nuclear antigen (PCNA). Within 24 h of resection, a new mass of PCNA-positive cells appeared at the filament tip. At 40 days post-resection, approximately half of resected tissue was replaced; and at 160 days post-resection, regeneration was nearly complete. Chemoreceptive neuroepithelial cells, identified by serotonin immunohistochemistry, were present in regenerates and established innervation by nerve fibres. Use of the transgenic zebrafish line Tg(fli1a:EGFP), in which the gill vasculature was labelled with enhanced green fluorescent protein, indicated that angiogenesis occurred during the regenerative process. Thus, the zebrafish is capable of substantive gill regeneration and replacement of respiratory chemoreceptors.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping