PUBLICATION
Feeding Entrainment of the Zebrafish Circadian Clock Is Regulated by the Glucocorticoid Receptor
- Authors
- Morbiato, E., Frigato, E., Dinarello, A., Maradonna, F., Facchinello, N., Argenton, F., Carnevali, O., Dalla Valle, L., Bertolucci, C.
- ID
- ZDB-PUB-191102-31
- Date
- 2019
- Source
- Cells 8(11): (Journal)
- Registered Authors
- Argenton, Francesco, Bertolucci, Cristiano, Carnevali, Oliana, Dalla Valle, Luisa, Facchinello, Nicola, Frigato, Elena
- Keywords
- Danio rerio, circadian clock, clock gene, entrainment, feeding, glucocorticoid receptors, metabolism, zebrafish
- MeSH Terms
-
- Animal Nutritional Physiological Phenomena/genetics
- Animals
- Animals, Genetically Modified
- Circadian Clocks/genetics
- Circadian Clocks/physiology*
- Circadian Rhythm/genetics
- Conditioning, Operant/physiology*
- Embryo, Nonmammalian
- Feeding Behavior/physiology*
- Motor Activity/physiology
- Photoperiod
- Receptors, Glucocorticoid/genetics
- Receptors, Glucocorticoid/physiology*
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 31671854 Full text @ Cells
Citation
Morbiato, E., Frigato, E., Dinarello, A., Maradonna, F., Facchinello, N., Argenton, F., Carnevali, O., Dalla Valle, L., Bertolucci, C. (2019) Feeding Entrainment of the Zebrafish Circadian Clock Is Regulated by the Glucocorticoid Receptor. Cells. 8(11):.
Abstract
Glucocorticoids (GCs) are steroid hormones mainly acting as key regulators of body homeostasis and stress responses. Their activities are primarily based on the binding to the GC receptor (GR), a member of the nuclear receptor family, that regulates tissue-specific sets of genes. GCs secretion follows a circadian rhythmicity with a peak linked to the animal's activity phase. In mammals, GCs are also implicated in feeding entrainment mechanisms as internal zeitgeber. Here, we investigated, by means of behavioural and molecular approaches, the circadian clock and its regulation by light and food in wild-type (WT) and null glucocorticoid receptor (gr-/-) zebrafish larvae, juveniles and adults. In both WT and gr-/- larvae and adults, behavioural activity and clock gene expression were entrained to the light-dark (LD) cycle and rhythmic in constant conditions. Differences in the pattern of clock genes' expression indicated a modulatory role of GCs. A significant role of Gr was detected in the feeding entrainment which was absent or markedly dampened in mutants. Furthermore, the expression of two clock-regulated genes involved in glucidic and lipidic metabolism was altered, highlighting the participation of GCs in metabolic processes also in fish. Taken together, our results confirmed the role of GC-mediated Gr signaling in the feeding entrainment in a non-mammalian species, the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping