PUBLICATION
Analysis of tail coiling activity of zebrafish (Danio rerio) embryos allows for the differentiation of neurotoxicants with different modes of action
- Authors
- Zindler, F., Beedgen, F., Brandt, D., Steiner, M., Stengel, D., Baumann, L., Braunbeck, T.
- ID
- ZDB-PUB-191015-6
- Date
- 2019
- Source
- Ecotoxicology and environmental safety 186: 109754 (Journal)
- Registered Authors
- Braunbeck, Thomas
- Keywords
- Acetylcholine esterase inhibitor, Heavy metals, Locomotor activity, Pesticides, Selective serotonin reuptake inhibitor, Spontaneous coiling movements
- MeSH Terms
-
- Animals
- Cadmium/toxicity
- Citalopram/toxicity
- Dichlorvos/toxicity
- Ecotoxicology/methods
- Embryo, Nonmammalian/drug effects*
- Embryonic Development
- Environmental Indicators*
- Fluoxetine/toxicity
- Movement/drug effects*
- Neurotoxicity Syndromes/etiology*
- Tail
- Toxicity Tests/methods*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 31606639 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zindler, F., Beedgen, F., Brandt, D., Steiner, M., Stengel, D., Baumann, L., Braunbeck, T. (2019) Analysis of tail coiling activity of zebrafish (Danio rerio) embryos allows for the differentiation of neurotoxicants with different modes of action. Ecotoxicology and environmental safety. 186:109754.
Abstract
In (eco)toxicology, there is a critical need for efficient methods to evaluate the neurotoxic potential of environmental chemicals. Recent studies proposed analysis of early coiling activity in zebrafish embryos as a powerful tool for the identification of neurotoxic compounds. In order to demonstrate that the analysis of early tail movements of zebrafish embryos allows for the discrimination of neurotoxicants acting via different mechanisms, the present study investigated the effects of four different neurotoxicants on the embryogenesis (fish embryo toxicity test) and early tail coiling movements of zebrafish embryos. Cadmium predominantly increased the frequency of tail coiling at the late pharyngula stage. Dichlorvos delayed embryonic development and caused convulsive tail movements resulting in prolonged duration of tail coils. Embryos exposed to teratogenic concentrations of fluoxetine and citalopram displayed absence of spontaneous tail movements at 24 h post-fertilization. In contrast, a non-teratogenic test concentration of citalopram decreased coiling frequency at multiple time points. Results demonstrated that the analysis of tail coiling movements of zebrafish embryos has the potential to discriminate neurotoxic compounds with different primary modes of action. In addition, chemical-induced effects on coiling activity were shown to potentially overlap with effects on embryogenesis. Further studies are needed to clarify the interplay of unspecific developmental toxicity of neurotoxic chemicals and effects resulting from specific neurotoxic mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping