PUBLICATION
Endocrine and local signaling interact to regulate spermatogenesis in zebrafish: Follicle-stimulating hormone, retinoic acid and androgens
- Authors
- Crespo, D., Assis, L.H.C., van de Kant, H.J.G., de Waard, S., Safian, D., Lemos, M.S., Bogerd, J., Schulz, R.W.
- ID
- ZDB-PUB-191011-22
- Date
- 2019
- Source
- Development (Cambridge, England) 146(21): (Journal)
- Registered Authors
- Bogerd, Jan, Schulz, Rüdiger W.
- Keywords
- 11-ketotestosterone, RNA sequencing, Retinoic acid, Spermatogenesis, Zebrafish
- Datasets
- GEO:GSE116611
- MeSH Terms
-
- Androgens/physiology*
- Animals
- Busulfan/chemistry
- Cell Differentiation/genetics
- Endocrine System/physiology*
- Feedback, Physiological
- Follicle Stimulating Hormone/physiology*
- Gene Expression Regulation, Developmental
- Male
- Mice
- Retinoids/physiology
- Signal Transduction*
- Spermatids/physiology
- Spermatocytes/physiology
- Spermatogenesis*
- Spermatogonia/physiology
- Testis/physiology
- Transgenes
- Tretinoin/physiology*
- Zebrafish
- PubMed
- 31597660 Full text @ Development
Citation
Crespo, D., Assis, L.H.C., van de Kant, H.J.G., de Waard, S., Safian, D., Lemos, M.S., Bogerd, J., Schulz, R.W. (2019) Endocrine and local signaling interact to regulate spermatogenesis in zebrafish: Follicle-stimulating hormone, retinoic acid and androgens. Development (Cambridge, England). 146(21):.
Abstract
Retinoic acid (RA) is critical for mammalian spermatogonia differentiation, and stimulates Stra8 expression, a gene required for meiosis. Certain fish species, including zebrafish, have lost the stra8 gene. While RA still seems important for spermatogenesis in fish, it is not known which stage(s) respond to RA or if its effects are integrated into the endocrine regulation of spermatogenesis. In zebrafish, RA promoted spermatogonia differentiation, supported androgen-stimulated meiosis and reduced spermatocyte and spermatid apoptosis. Follicle-stimulating hormone (Fsh) stimulated RA production. Expressing a dominant-negative RA receptor variant in germ cells clearly disturbed spermatogenesis but meiosis and spermiogenesis still took place although sperm quality was low in 6 months-old adults. This condition also activated Leydig cells. Three months later, spermatogenesis apparently had recovered, but doubling of testis weight demonstrated hypertrophy, apoptosis/DNA damage among spermatids was high and sperm quality remained low. We conclude that RA signaling is important for zebrafish spermatogenesis but is not of critical relevance. Since Fsh stimulates androgen and RA production, germ cell-mediated, RA-dependent reduction of Leydig cell activity may form a hitherto unknown intratesticular negative feedback loop.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping