PUBLICATION

Vegfc/d-dependent regulation of the lymphatic vasculature during cardiac regeneration is influenced by injury context

Authors
Vivien, C.J., Pichol-Thievend, C., Sim, C.B., Smith, J.B., Bower, N.I., Hogan, B.M., Hudson, J.E., Francois, M., Porrello, E.R.
ID
ZDB-PUB-190828-13
Date
2019
Source
NPJ Regenerative medicine   4: 18 (Journal)
Registered Authors
Hogan, Ben M.
Keywords
Angiogenesis, Cardiac regeneration
Datasets
GEO:GSE133130, GEO:GSE133131, GEO:GSE133129
MeSH Terms
none
PubMed
31452940 Full text @ NPJ Regen Med
Abstract
The lymphatic vasculature mediates essential physiological functions including fluid homeostasis, lipid and hormone transport, and immune cell trafficking. Recent studies have suggested that promoting lymphangiogenesis enhances cardiac repair following injury, but it is unknown whether lymphangiogenesis is required for cardiac regeneration. Here, we describe the anatomical distribution, regulation, and function of the cardiac lymphatic network in a highly regenerative zebrafish model system using transgenic reporter lines and loss-of-function approaches. We show that zebrafish lacking functional vegfc and vegfd signaling are devoid of a cardiac lymphatic network and display cardiac hypertrophy in the absence of injury, suggesting a role for these vessels in cardiac tissue homeostasis. Using two different cardiac injury models, we report a robust lymphangiogenic response following cryoinjury, but not following apical resection injury. Although the majority of mutants lacking functional vegfc and vegfd signaling were able to mount a full regenerative response even in the complete absence of a cardiac lymphatic vasculature, cardiac regeneration was severely impaired in a subset of mutants, which was associated with heightened pro-inflammatory cytokine signaling. These findings reveal a context-dependent requirement for the lymphatic vasculature during cardiac growth and regeneration.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping