PUBLICATION
Effect of pH on the toxicity of fumonisins towards the RTL-W1 cell line and zebrafish (Danio rerio) embryos
- Authors
- du Plessis, B., Regnier, T., Combrinck, S., Heinrich, P., Braunbeck, T.
- ID
- ZDB-PUB-190701-20
- Date
- 2019
- Source
- Toxicology letters 313: 101-107 (Journal)
- Registered Authors
- Braunbeck, Thomas
- Keywords
- Danio rerio, Fumonisins, RTL-W1, Toxicity, Zebrafish, pH
- MeSH Terms
-
- Animals
- Cell Line
- Cell Survival/drug effects
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Fumonisins/toxicity*
- Hydrogen-Ion Concentration
- Hydrolysis
- Inhibitory Concentration 50
- Lethal Dose 50
- Liver/drug effects*
- Liver/pathology
- Oncorhynchus mykiss*
- Risk Assessment
- Zebrafish/embryology*
- PubMed
- 31254606 Full text @ Toxicol. Lett.
Citation
du Plessis, B., Regnier, T., Combrinck, S., Heinrich, P., Braunbeck, T. (2019) Effect of pH on the toxicity of fumonisins towards the RTL-W1 cell line and zebrafish (Danio rerio) embryos. Toxicology letters. 313:101-107.
Abstract
Fumonisins are common contaminants of maize. The neutral red assay, using the RTL-W1 trout liver cell line, and the fish embryo test (FET), using zebrafish, were selected to assess the effect of pH on the cytotoxicity, acute toxicity and teratogenicity of fumonisin B1 (FB1). The results demonstrated that FB1 exerts low cytotoxicity towards RTL-W1 cells without pH adjustment (IC50 1 746 μM), and no cytotoxicity after pH-adjustment to physiological conditions. The LC50 value for FB1 in the FET (1 058 μM at 48 h) confirmed low acute toxicity. Adjusting the pH to physiological conditions reduced the acute toxicity of FB1 towards zebrafish embryos, emphasising the importance of acidity/basicity of the medium in toxicity testing. Hydrolysed FB1 was less toxic than FB1 (LC50 2 690 μM at 48 h), and neither were teratogenic towards zebrafish embryos. Results confirm that the FET may account for effects not observable in cell cultures.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping