PUBLICATION
Single cell dynamics of embryonic muscle progenitor cells in zebrafish
- Authors
- Sharma, P., Ruel, T.D., Kocha, K.M., Liao, S., Huang, P.
- ID
- ZDB-PUB-190701-18
- Date
- 2019
- Source
- Development (Cambridge, England) 146(14): (Journal)
- Registered Authors
- Huang, Peng, Kocha, Katrinka
- Keywords
- Dermomyotome, Extracellular matrix, In vivo imaging, Muscle progenitor cells, Somite, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Differentiation
- Collagen/genetics
- Collagen/metabolism
- Embryo, Nonmammalian
- Embryonic Stem Cells/cytology
- Embryonic Stem Cells/physiology
- Kinetics
- Muscle Development/physiology
- Muscle Fibers, Skeletal/physiology
- Myoblasts/cytology*
- Myoblasts/physiology*
- PAX2 Transcription Factor/genetics
- PAX2 Transcription Factor/metabolism
- Regeneration/genetics
- Single-Cell Analysis*
- Time-Lapse Imaging
- Zebrafish*/embryology
- Zebrafish*/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 31253635 Full text @ Development
Citation
Sharma, P., Ruel, T.D., Kocha, K.M., Liao, S., Huang, P. (2019) Single cell dynamics of embryonic muscle progenitor cells in zebrafish. Development (Cambridge, England). 146(14):.
Abstract
Muscle stem cells hold a great therapeutic potential in regenerating damaged muscles. However, the in vivo behavior of muscle stem cells during muscle growth and regeneration is still poorly understood. Using zebrafish as a model, we describe the in vivo dynamics and function of embryonic muscle progenitor cells (MPCs) in the dermomyotome. These cells are located in a superficial layer external to muscle fibers and express many extracellular matrix (ECM) genes including col1a2 Utilizing a new col1a2 transgenic line, we show that col1a2+ MPCs display a ramified morphology with dynamic cellular processes. Cell lineage tracing demonstrates that col1a2+ MPCs contribute to new myofibers during both normal muscle growth as well as muscle regeneration. Combination of live imaging and single cell clonal analysis reveals a highly-choreographed process of muscle regeneration. Activated col1a2+ MPCs change from the quiescent ramified morphology to a polarized and elongated morphology and generate daughter cells that fuse with existing myofibers. Partial depletion of col1a2+ MPCs severely compromises muscle regeneration. Our work provides a dynamic view of embryonic muscle progenitor cells during zebrafish muscle growth and regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping