ZFIN ID: ZDB-PUB-190701-18
Single cell dynamics of embryonic muscle progenitor cells in zebrafish
Sharma, P., Ruel, T.D., Kocha, K.M., Liao, S., Huang, P.
Date: 2019
Source: Development (Cambridge, England)   146(14): (Journal)
Registered Authors: Huang, Peng, Kocha, Katrinka
Keywords: Dermomyotome, Extracellular matrix, In vivo imaging, Muscle progenitor cells, Somite, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation
  • Collagen/genetics
  • Collagen/metabolism
  • Embryo, Nonmammalian
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/physiology
  • Kinetics
  • Muscle Development/physiology
  • Muscle Fibers, Skeletal/physiology
  • Myoblasts/cytology*
  • Myoblasts/physiology*
  • PAX2 Transcription Factor/genetics
  • PAX2 Transcription Factor/metabolism
  • Regeneration/genetics
  • Single-Cell Analysis*
  • Time-Lapse Imaging
  • Zebrafish*/embryology
  • Zebrafish*/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 31253635 Full text @ Development
Muscle stem cells hold a great therapeutic potential in regenerating damaged muscles. However, the in vivo behavior of muscle stem cells during muscle growth and regeneration is still poorly understood. Using zebrafish as a model, we describe the in vivo dynamics and function of embryonic muscle progenitor cells (MPCs) in the dermomyotome. These cells are located in a superficial layer external to muscle fibers and express many extracellular matrix (ECM) genes including col1a2 Utilizing a new col1a2 transgenic line, we show that col1a2+ MPCs display a ramified morphology with dynamic cellular processes. Cell lineage tracing demonstrates that col1a2+ MPCs contribute to new myofibers during both normal muscle growth as well as muscle regeneration. Combination of live imaging and single cell clonal analysis reveals a highly-choreographed process of muscle regeneration. Activated col1a2+ MPCs change from the quiescent ramified morphology to a polarized and elongated morphology and generate daughter cells that fuse with existing myofibers. Partial depletion of col1a2+ MPCs severely compromises muscle regeneration. Our work provides a dynamic view of embryonic muscle progenitor cells during zebrafish muscle growth and regeneration.