PUBLICATION

Slc25a17 acts as a peroxisomal coenzyme A transporter and regulates multiorgan development in zebrafish

Authors
Kim, Y.I., Nam, I.K., Lee, D.K., Bhandari, S., Charton, L., Kwak, S., Lim, J.Y., Hong, K., Kim, S.J., Lee, J.N., Kwon, S.W., So, H.S., Linka, N., Park, R., Choe, S.K.
ID
ZDB-PUB-190613-5
Date
2019
Source
Journal of Cellular Physiology   235(1): 151-165 (Journal)
Registered Authors
Bhandari, Sushil, Choe, Seong-Kyu, Kim, Yong-Il
Keywords
coenzyme A transporter, peroxisomes, solute carrier family 25 member 17, swim bladder, zebrafish
MeSH Terms
  • Air Sacs/growth & development
  • Air Sacs/metabolism
  • Amino Acid Sequence
  • Animals
  • Coenzyme A/genetics
  • Coenzyme A/metabolism*
  • Conserved Sequence
  • Evolution, Molecular
  • Gene Expression Regulation, Developmental/physiology*
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Zebrafish
PubMed
31187491 Full text @ J. Cell. Physiol.
Abstract
Slc25a17 is known as a peroxisomal solute carrier, but the in vivo role of the protein has not been demonstrated. We found that the zebrafish genome contains two slc25a17 genes that function redundantly, but additively. Notably, peroxisome function in slc25a17 knockdown embryos is severely compromised, resulting in an altered lipid composition. Along the defects found in peroxisome-associated phenotypic presentations, we highlighted that development of the swim bladder is also highly dependent on Slc25a17 function. As Slc25a17 showed substrate specificity towards coenzyme A (CoA), injecting CoA, but not NAD+ , rescued the defective swim bladder induced by slc25a17 knockdown. These results indicated that Slc25a17 acts as a CoA transporter, involved in the maintenance of functional peroxisomes that are essential for the development of multiple organs during zebrafish embryogenesis. Given high homology in protein sequences, the role of zebrafish Slc25a17 may also be applicable to the mammalian system.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping