ZFIN ID: ZDB-PUB-190612-2
Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species
Wu, S.H., Chou, H.Y., Liu, P.C., Wu, J.L., Gong, H.Y.
Date: 2019
Source: Biochemical and Biophysical Research Communications   515(4): 706-711 (Journal)
Registered Authors: Gong, Hong-Yi, Wu, Jen-Leih
Keywords: Antimicrobial peptide, Bactericidal, Bacteriostatic, Granulin, Tilapia, Vibrio
MeSH Terms:
  • Alternative Splicing
  • Animals
  • Anti-Bacterial Agents/pharmacology*
  • Antimicrobial Cationic Peptides/pharmacology*
  • Granulins
  • Hemolysis
  • Hydrogen-Ion Concentration
  • Microscopy, Electron, Transmission
  • Sheep
  • Temperature
  • Tilapia/metabolism*
  • Vibrio/drug effects*
  • Vibrio Infections/drug therapy*
PubMed: 31182280 Full text @ Biochem. Biophys. Res. Commun.
ABSTRACT
In our previous study, the novel GRN-41 peptide generated from alternative splicing of the Mozambique tilapia PGRN1 gene was identified to be a potent peptide that protected against V. vulnificus in the transgenic zebrafish model by modulating innate immune-related genes. In this study, the anti-bacterial activities of synthetic Mozambique tilapia GRN-41 peptide (OmGRN-41) against various bacterial pathogens were investigated. The results showed that OmGRN-41 had bactericidal activity against Vibrio species, including V. vulnificus, V. alginolyticus, and V. harveyi, but exhibited bacteriostatic activity against V. parahaemolyticus. OmGRN-41 maintained bactericidal activity (64 μM) against V. vulnificus at pH 2 to pH 10 or after heat treatment for 1 h at high temperatures between 40 °C and 100 °C. TEM observations revealed that the outer membrane of V. vulnificus was disrupted by OmGRN-41, leading to morphological rupture and loss of cytoplasmic contents. Additionally, little hemolytic activity against tilapia and sheep erythrocytes was detected after treatment with 128 μM OmGRN-41. OmGRN-41 can effectively enhance the survival of Nile tilapia infected by V. vulnificus. Our results suggest that the OmGRN-41 is a novel antimicrobial peptide possessing bactericidal activity, especially against Vibrio species. These results indicate that OmGRN-41 can be applied in human Vibriosis treatment and has the potential to defend against Vibrio spp. infection in critical aquaculture organisms.
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