PUBLICATION

Network Properties Revealed during Multi-Scale Calcium Imaging of Seizure Activity in Zebrafish

Authors
Liu, J., Baraban, S.C.
ID
ZDB-PUB-190322-7
Date
2019
Source
eNeuro   6(1): (Journal)
Registered Authors
Baraban, Scott
Keywords
epilepsy, fast confocal, neuronal networks, synchronization, whole-brain imaging, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Brain/pathology
  • Brain/physiopathology*
  • Calcium/metabolism*
  • Cortical Synchronization
  • Epilepsy/metabolism
  • Epilepsy/physiopathology
  • Microphthalmia-Associated Transcription Factor/genetics
  • Microphthalmia-Associated Transcription Factor/metabolism
  • Neural Pathways/pathology
  • Neural Pathways/physiopathology
  • Pentylenetetrazole
  • Seizures/pathology
  • Seizures/physiopathology*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
30895220 Full text @ eNeuro
Abstract
Seizures are characterized by hypersynchronization of neuronal networks. Understanding these networks could provide a critical window for therapeutic control of recurrent seizure activity, i.e., epilepsy. However, imaging seizure networks has largely been limited to microcircuits in vitro or small "windows" in vivo. Here, we combine fast confocal imaging of genetically encoded calcium indicator (GCaMP)-expressing larval zebrafish with local field potential (LFP) recordings to study epileptiform events at whole-brain and single-neuron levels in vivo. Using an acute seizure model (pentylenetetrazole, PTZ), we reliably observed recurrent electrographic ictal-like events associated with generalized activation of all major brain regions and uncovered a well-preserved anterior-to-posterior seizure propagation pattern. We also examined brain-wide network synchronization and spatiotemporal patterns of neuronal activity in the optic tectum microcircuit. Brain-wide and single-neuronal level analysis of PTZ-exposed and 4-aminopyridine (4-AP)-exposed zebrafish revealed distinct network dynamics associated with seizure and non-seizure hyperexcitable states, respectively. Neuronal ensembles, comprised of coactive neurons, were also uncovered during interictal-like periods. Taken together, these results demonstrate that macro- and micro-network calcium motifs in zebrafish may provide a greater understanding of epilepsy.
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping