The negative regulation of piscine CD44c in viral and bacterial infection

Cao, L., Wu, X.M., Nie, P., Chang, M.X.
Developmental and comparative immunology   96: 135-143 (Journal)
Registered Authors
Cao, Lu, Chang, Mingxian, Nie, Pin, Wu, Xiaoman
Antibacterial molecules, Antiviral genes, Bacterial infection, Inflammatory cytokines, MHC genes, Negative regulation, Viral infection, Zebrafish CD44
MeSH Terms
  • Alternative Splicing/immunology
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cytokines/immunology
  • Cytokines/metabolism
  • Edwardsiella/immunology
  • Edwardsiella/pathogenicity
  • Enterobacteriaceae Infections/immunology*
  • Enterobacteriaceae Infections/microbiology
  • Fish Diseases/immunology*
  • Fish Diseases/microbiology
  • Hyaluronan Receptors/chemistry
  • Hyaluronan Receptors/genetics
  • Hyaluronan Receptors/immunology*
  • Immunity, Innate
  • Larva/immunology
  • Larva/metabolism
  • Phylogeny
  • Protein Isoforms/chemistry
  • Protein Isoforms/genetics
  • Protein Isoforms/immunology
  • Rhabdoviridae/immunology
  • Rhabdoviridae/pathogenicity
  • Rhabdoviridae Infections/immunology*
  • Rhabdoviridae Infections/virology
  • Structure-Activity Relationship
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • Zebrafish/metabolism
  • Zebrafish/virology
30885554 Full text @ Dev. Comp. Immunol.
CD44 gene is a cell surface receptor which undergoes complex alternative splicing and extensive post-translational modifications. Although many studies have showed that CD44 is involved in the process of host defense, the function of piscine CD44 in antibacterial or antiviral defense response remains unclear. In the present study, we report the functional characterization of zebrafish CD44c, which is more similar to CD44b antigen isoforms rather than CD44a based on amino acid composition and phylogenetic analysis. The expression of zebrafish CD44c was inducible in response to bacterial and viral infections. During SVCV infection, the in vivo studies revealed that CD44c overexpression led to the increased virus loads and decreased survival rate. The attenuated response by zebrafish CD44c in response to SVCV infection were characterized by the impaired production of inflammatory cytokines and the impaired expressions of IFNs, IFN-stimulated genes, MHC class I and II genes. During Edwardsiella piscicida infection, the overexpression of zebrafish CD44c facilitated bacterial growth and dissemination, but did not impact on larvae survival. The detrimental role of CD44c in host defense against E. piscicida infection was supported by a decreased production of several antibacterial molecules including defbl2, defbl3, NK-lysin and RNase3. All together, these results firstly demonstrate the negative regulation of piscine CD44c in viral and bacterial infection.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes