PUBLICATION
Cellular responses to ionizing radiation change quickly over time during early development in zebrafish
- Authors
- Honjo, Y., Ichinohe, T.
- ID
- ZDB-PUB-190223-9
- Date
- 2019
- Source
- Cell biology international 43(5): 516-527 (Journal)
- Registered Authors
- Honjo, Yasuko
- Keywords
- early development, radiation, zebrafish
- MeSH Terms
-
- Animals
- Cyclin-Dependent Kinase Inhibitor p21/radiation effects
- DNA Damage
- Embryo, Nonmammalian/metabolism
- Embryonic Development/radiation effects*
- Gamma Rays/adverse effects
- Radiation, Ionizing
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/radiation effects
- PubMed
- 30791195 Full text @ Cell Biol. Int.
Citation
Honjo, Y., Ichinohe, T. (2019) Cellular responses to ionizing radiation change quickly over time during early development in zebrafish. Cell biology international. 43(5):516-527.
Abstract
Animal cells constantly receive information about and respond to environmental factors, including ionizing radiation. Although it is crucial for a cell to repair radiation-induced DNA damage to ensure survival, cellular responses to radiation exposure during early embryonic development remain unclear. In this study, we analyzed the effects of ionizing radiation in zebrafish embryos and found that radiation-induced γH2AX foci formation and cell cycle arrest did not occur until the gastrula stage, despite the presence of major DNA repair-related gene transcripts, passed on as maternal factors. Interestingly, P21/WAF1 accumulation began ∼ 6 h post-fertilization, although p21 mRNA was upregulated by irradiation at 2 or 4 h post-fertilization. These results suggest that the cellular responses of zebrafish embryos at 2 or 4 h post-fertilization to radiation failed to overcome P21 protein accumulation and further signaling. Regulation of P21/WAF1 protein stabilization appears to be a key factor in the response to genotoxins during early embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping