PUBLICATION

BMP signaling is required for nkx2.3-positive pharyngeal pouch progenitor specification in zebrafish

Authors
Li, L., Ning, G., Yang, S., Yan, Y., Cao, Y., Wang, Q.
ID
ZDB-PUB-190215-16
Date
2019
Source
PLoS Genetics   15: e1007996 (Journal)
Registered Authors
Wang, Qiang
Keywords
none
MeSH Terms
  • Animals
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Pharynx/embryology
  • Pharynx/metabolism
  • Cell Lineage/genetics
  • Cell Lineage/physiology
  • Bone Morphogenetic Protein 2/genetics
  • Bone Morphogenetic Protein 2/metabolism*
  • Animals, Genetically Modified
  • Smad Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Platelet-Derived Growth Factor/genetics
  • Platelet-Derived Growth Factor/metabolism
  • Mutation
  • Signal Transduction
  • Endoderm/embryology
  • Endoderm/metabolism
  • Somites/embryology
  • Somites/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
(all 28)
PubMed
30763319 Full text @ PLoS Genet.
Abstract
Pharyngeal pouches, a series of outpocketings that bud from the foregut endoderm, are essential to the formation of craniofacial skeleton as well as several important structures like parathyroid and thymus. However, whether pharyngeal pouch progenitors exist in the developing gut tube remains unknown. Here, taking advantage of cell lineage tracing and transgenic ablation technologies, we identified a population of nkx2.3+ pouch progenitors in zebrafish embryos and demonstrated an essential requirement of ectodermal BMP2b for their specification.At early somite stages, nkx2.3+ cells located at lateral region of pharyngeal endoderm give rise to the pouch epithelium except a subpopulation expressing pdgfαa rather than nkx2.3. A small-scale screen of chemical inhibitors reveals that BMP signaling is necessary to specify these progenitors. Loss-of-function analyses show that BMP2b, expressed in the pharyngeal ectoderm, actives Smad effectors in endodermal cells to induce nkx2.3+ progenitors. Collectively, our study provides in vivo evidence for the existence of pouch progenitors and highlights the importance of BMP2b signaling in progenitor specification.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f1TgTransgenic Insertion
    fb7TgTransgenic Insertion
      s870TgTransgenic Insertion
        ta72a
          		Point Mutation
          w30TgTransgenic Insertion
            w64TgTransgenic Insertion
              y1TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                bmp2bMO1-bmp2bMRPHLNO
                bmp4MO1-bmp4MRPHLNO
                bmp7aMO1-bmp7aMRPHLNO
                sox32MO2-sox32MRPHLNO
                1 - 4 of 4
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                Fish
                No data available
                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                ZsYellowEFGZsYellow
                1 - 3 of 3
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                Mapping
                No data available
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                Citation
                Li, L., Ning, G., Yang, S., Yan, Y., Cao, Y., Wang, Q. (2019) BMP signaling is required for nkx2.3-positive pharyngeal pouch progenitor specification in zebrafish. PLoS Genetics. 15:e1007996.