PUBLICATION
Generation of a zebrafish SWATH-MS spectral library to quantify 10,000 proteins
- Authors
- Blattmann, P., Stutz, V., Lizzo, G., Richard, J., Gut, P., Aebersold, R.
- ID
- ZDB-PUB-190213-7
- Date
- 2019
- Source
- Scientific data 6: 190011 (Journal)
- Registered Authors
- Gut, Philipp, Richard, Joy
- Keywords
- none
- MeSH Terms
-
- Animals
- Mass Spectrometry
- Peptide Library*
- Proteomics/methods*
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 30747917 Full text @ Sci Data
Citation
Blattmann, P., Stutz, V., Lizzo, G., Richard, J., Gut, P., Aebersold, R. (2019) Generation of a zebrafish SWATH-MS spectral library to quantify 10,000 proteins. Scientific data. 6:190011.
Abstract
Sequential window acquisition of all theoretical mass spectra (SWATH-MS) requires a spectral library to extract quantitative measurements from the mass spectrometry data acquired in data-independent acquisition mode (DIA). Large combined spectral libraries containing SWATH assays have been generated for humans and several other organisms, but so far no publicly available library exists for measuring the proteome of zebrafish, a rapidly emerging model system in biomedical research. Here, we present a large zebrafish SWATH spectral library to measure the abundance of 104,185 proteotypic peptides from 10,405 proteins. The library includes proteins expressed in 9 different zebrafish tissues (brain, eye, heart, intestine, liver, muscle, ovary, spleen, and testis) and provides an important new resource to quantify 40% of the protein-coding zebrafish genes. We employ this resource to quantify the proteome across brain, muscle, and liver and characterize divergent expression levels of paralogous proteins in different tissues. Data are available via ProteomeXchange (PXD010876, PXD010869) and SWATHAtlas (PASS01237).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping