PUBLICATION

A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Authors
Hadzhiev, Y., Qureshi, H.K., Wheatley, L., Cooper, L., Jasiulewicz, A., Van Nguyen, H., Wragg, J.W., Poovathumkadavil, D., Conic, S., Bajan, S., Sik, A., Hutvàgner, G., Tora, L., Gambus, A., Fossey, J.S., Müller, F.
ID
ZDB-PUB-190212-4
Date
2019
Source
Nature communications   10: 691 (Journal)
Registered Authors
Hadzhiev, Yavor, Müller, Ferenc
Keywords
none
MeSH Terms
  • Blastula/diagnostic imaging
  • Blastula/physiology
  • Cell Cycle/genetics*
  • Cell Cycle/physiology
  • Blastocyst/physiology
  • Cell Nucleus/physiology
  • Chromatin
  • Four-Dimensional Computed Tomography
  • Zygote/physiology
  • Transcriptome/genetics
  • Spatio-Temporal Analysis
  • Zebrafish/genetics
  • Gene Expression Regulation, Developmental/genetics*
  • Gene Expression Regulation, Developmental/physiology
  • Genome/genetics*
  • Genome/physiology
  • MicroRNAs
  • Animals
  • Cell Division
  • S Phase/physiology
  • Models, Animal
  • Chromosomes
  • Transcription, Genetic/genetics*
  • Transcription, Genetic/physiology
(all 24)
PubMed
30741925 Full text @ Nat. Commun.
Abstract
Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
in1TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Fish
    No data available
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab4-polr2monoclonal
      		IgGMouse
      Ab5-polr2polyclonal
        		IgGRabbit
        Ab6-polr2monoclonal
          		Mouse
          Ab51-h3polyclonal
            		IgGRabbit
            1 - 4 of 4
            Show
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            mRFP1EFGmRFP1
            1 - 1 of 1
            Show
            Mapping
            No data available
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            Citation
            Hadzhiev, Y., Qureshi, H.K., Wheatley, L., Cooper, L., Jasiulewicz, A., Van Nguyen, H., Wragg, J.W., Poovathumkadavil, D., Conic, S., Bajan, S., Sik, A., Hutvàgner, G., Tora, L., Gambus, A., Fossey, J.S., Müller, F. (2019) A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation. Nature communications. 10:691.