PUBLICATION
Propyl gallate inhibits hepatocellular carcinoma cell growth through the induction of ROS and the activation of autophagy
- Authors
- Wei, P.L., Huang, C.Y., Chang, Y.J.
- ID
- ZDB-PUB-190118-8
- Date
- 2019
- Source
- PLoS One 14: e0210513 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Apoptosis Regulatory Proteins/metabolism
- Autophagy/drug effects*
- Autophagy-Related Proteins/metabolism
- Carcinoma, Hepatocellular/drug therapy*
- Carcinoma, Hepatocellular/metabolism
- Carcinoma, Hepatocellular/pathology
- Cell Line, Tumor
- Cell Proliferation/drug effects
- Humans
- Liver Neoplasms/drug therapy*
- Liver Neoplasms/metabolism
- Liver Neoplasms/pathology
- Propyl Gallate/pharmacology*
- Reactive Oxygen Species/metabolism*
- Xenograft Model Antitumor Assays/methods*
- Zebrafish
- PubMed
- 30653551 Full text @ PLoS One
Citation
Wei, P.L., Huang, C.Y., Chang, Y.J. (2019) Propyl gallate inhibits hepatocellular carcinoma cell growth through the induction of ROS and the activation of autophagy. PLoS One. 14:e0210513.
Abstract
The poor prognosis of hepatocellular carcinoma (HCC) has been attributed to a high frequency of tumor metastasis and recurrence even after successful surgical resection. With less than 30% of patients benefiting from curative treatment, alternative treatment regimens for patients with advanced HCC are needed. Propyl gallate (PG), a synthetic antioxidant used in preserving food and medicinal preparations, has been shown to induce cancer cell death, but the anticancer effects of PG in HCC are unclear. In the present study, we demonstrated that PG inhibited HCC cell proliferation in vitro and in zebrafish models in vivo in a dose- and time-dependent manner. PG also induced cell apoptosis and increased the number of necrotic cells in a time- and dose-dependent manner as determined using a high-content analysis system. We found that PG also increased the intracellular levels of superoxide and reactive oxidative stress as well as the formation of autophagosomes and lysosomes. Regarding the molecular mechanism, PG did not alter the levels of autophagy-related 5 (ATG5), ATG5/12 or Beclin-1 but increased the rate of the LC3-I to LC3-II conversion, suggesting autophagy induction. PG exposure increased the levels of the pro-apoptotic proteins cleaved caspase-3, cleaved PARP, Bax, and Bad and a decreased level of the anti-apoptotic protein Bcl-2. In conclusion, we demonstrate that PG inhibits HCC cell proliferation through enhanced ROS production and autophagy activation. Finally, PG-treated cells induced cell apoptosis and may be a new candidate for HCC therapy.
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
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