PUBLICATION

Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer

Authors
Dasgupta, A., Jacob, A.E., Amack, J.D.
ID
ZDB-PUB-190108-4
Date
2018
Source
Bio-protocol   8(22): (Journal)
Registered Authors
Amack, Jeffrey, Dasgupta, Agnik
Keywords
3D reconstructions, Dorsal forerunner cells, Kupffer’s vesicle, Left-right organizer, Morphometric analysis, Mosaic labeling, Single cell, Zebrafish embryo
MeSH Terms
none
PubMed
30613762 Full text @ Bio Protoc
Abstract
A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors during LRO formation. To address this, we developed a Cre-loxP based method to mosaically label precursor cells, called dorsal forerunner cells, that give rise to the zebrafish LRO known as Kupffer's vesicle. This methodology allows lineage tracing, 3-dimensional (3D) reconstruction and morphometric analysis of single LRO cells in living embryos. The ability to visualize and quantify individual LRO cell dynamics provides an opportunity to advance our understanding of LRO development, and in a broader sense, investigate the interplay between intrinsic biochemical mechanisms and extrinsic mechanical forces that drive morphogenesis of epithelial tissues.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping