PUBLICATION
Possible involvement of Fas/FasL-dependent apoptotic pathway in α-bisabolol induced cardiotoxicity in zebrafish embryos
- Authors
- Jin, M., Xiao, Z., Zhang, S., Men, X., Li, X., Zhang, B., Zhou, T., Hsiao, C.D., Liu, K.
- ID
- ZDB-PUB-181216-10
- Date
- 2018
- Source
- Chemosphere 219: 557-566 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- Apoptosis, Cardiac developmental toxicity, Fas/FasL, Zebrafish, α-Bisabolol
- MeSH Terms
-
- Animals
- Apoptosis
- Cardiotoxicity/etiology*
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Fas Ligand Protein/metabolism
- Heart Defects, Congenital/chemically induced*
- Sesquiterpenes/toxicity*
- Zebrafish/embryology
- Zebrafish/metabolism
- fas Receptor/metabolism
- PubMed
- 30553216 Full text @ Chemosphere
Citation
Jin, M., Xiao, Z., Zhang, S., Men, X., Li, X., Zhang, B., Zhou, T., Hsiao, C.D., Liu, K. (2018) Possible involvement of Fas/FasL-dependent apoptotic pathway in α-bisabolol induced cardiotoxicity in zebrafish embryos. Chemosphere. 219:557-566.
Abstract
α-Bisabolol, an unsaturated monocyclic sesquiterpene alcohol, is a common ingredient in many pharmaceuticals and personal care products (PPCPs). Despite being widely used, little is known about its toxic effects on organisms and aquatic environment. In this study, we investigated the developmental toxicity of α-Bisabolol, especially its effects on the cardiac development using zebrafish embryos as a model. Embryos at 4 h post-fertilization (hpf) were exposed to 10, 30, 50, 70, 90, and 100 μM α-Bisabolol until 144 hpf. α-Bisabolol caused phenotypic defects and the most striking one is the heart malformation. Treatment of α-Bisabolol significantly increased the cardiac malformation rate, the SV-BA distance, as well as the pericardial edema area, and reduced heart rate in a concentration-dependent manner. Notably, considerable numbers of apoptotic cells were mainly observed in the heart region of zebrafish treated with α-Bisabolol. Further study on α-Bisabolol induced apoptosis in the zebrafsh heart suggested that an activation of Fas/FasL-dependent apoptotic pathway. Taken together, our study investigated the cardiotoxicity of α-Bisabolol on zebrafish embryonic development and its underlying molecular mechanism, shedding light on the full understanding of α-Bisabolol toxicity on living organisms and its environmental impact.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping