PUBLICATION

Brain-wide circuit interrogation at the cellular level guided by online analysis of neuronal function

Authors
Vladimirov, N., Wang, C., Höckendorf, B., Pujala, A., Tanimoto, M., Mu, Y., Yang, C.T., Wittenbach, J.D., Freeman, J., Preibisch, S., Koyama, M., Keller, P.J., Ahrens, M.B.
ID
ZDB-PUB-181207-11
Date
2018
Source
Nature Methods   15: 1117-1125 (Journal)
Registered Authors
Ahrens, Misha, Keller, Philipp, Vladimirov, Nikita, Yang, Chao-Tsung
Keywords
none
MeSH Terms
  • Animals
  • Behavior, Animal/physiology*
  • Brain/cytology
  • Brain/physiology*
  • Brain Mapping/methods*
  • Larva/physiology*
  • Neural Pathways
  • Neurons/cytology
  • Neurons/physiology*
  • Online Systems*
  • Swimming
  • Zebrafish/physiology*
PubMed
30504888 Full text @ Nat. Methods
Abstract
Whole-brain imaging allows for comprehensive functional mapping of distributed neural pathways, but neuronal perturbation experiments are usually limited to targeting predefined regions or genetically identifiable cell types. To complement whole-brain measures of activity with brain-wide manipulations for testing causal interactions, we introduce a system that uses measured activity patterns to guide optical perturbations of any subset of neurons in the same fictively behaving larval zebrafish. First, a light-sheet microscope collects whole-brain data that are rapidly analyzed by a distributed computing system to generate functional brain maps. On the basis of these maps, the experimenter can then optically ablate neurons and image activity changes across the brain. We applied this method to characterize contributions of behaviorally tuned populations to the optomotor response. We extended the system to optogenetically stimulate arbitrary subsets of neurons during whole-brain imaging. These open-source methods enable delineating the contributions of neurons to brain-wide circuit dynamics and behavior in individual animals.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes