PUBLICATION
Hypoxia Delays Oligodendrocyte Progenitor Cell Migration and Myelin Formation by Suppressing Bmp2b Signaling in Larval Zebrafish
- Authors
- Yang, L.Q., Chen, M., Zhang, J.L., Ren, D.L., Hu, B.
- ID
- ZDB-PUB-181020-26
- Date
- 2018
- Source
- Frontiers in Cellular Neuroscience 12: 348 (Journal)
- Registered Authors
- Hu, Bing
- Keywords
- bmp2b, differentiation, hypoxia, myelination, oligodendrocyte, zebrafish
- MeSH Terms
- none
- PubMed
- 30337858 Full text @ Front. Cell. Neurosci.
Citation
Yang, L.Q., Chen, M., Zhang, J.L., Ren, D.L., Hu, B. (2018) Hypoxia Delays Oligodendrocyte Progenitor Cell Migration and Myelin Formation by Suppressing Bmp2b Signaling in Larval Zebrafish. Frontiers in Cellular Neuroscience. 12:348.
Abstract
Hypoxia in newborns tends to result in developmental deficiencies in the white matter of the brain. As previous studies of the effects of hypoxia on neuronal development in rodents and human infants have been unable to use in vivo imaging, insight into the dynamic development of oligodendrocytes (OLs) in the central nervous system under hypoxia is limited. Here, we developed a visual model to study OL development using sublethal postnatal hypoxia in zebrafish larvae. We observed that hypoxia significantly suppressed OL progenitor cell migration toward the dorsum using in vivo imaging. Further, we found that hypoxia affected myelination, as indicated by thinner myelin sheaths and by a downregulation of myelin basic protein expression. Bmp2b protein expression was also significantly downregulated following hypoxia onset. Using gain of function and loss of function experiments, we demonstrated that the Bmp2b protein was associated with the regulation of OL development. Thus, our work provides a visual hypoxia model within which to observe OL development in vivo, and reveals the underlying mechanisms involved in these processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping