PUBLICATION
Scale-invariant patterning by size-dependent inhibition of Nodal signalling
- Authors
- Almuedo-Castillo, M., Bläßle, A., Mörsdorf, D., Marcon, L., Soh, G.H., Rogers, K.W., Schier, A.F., Müller, P.
- ID
- ZDB-PUB-180801-8
- Date
- 2018
- Source
- Nature cell biology 20(9): 1032-1042 (Journal)
- Registered Authors
- Müller, Patrick, Rogers, Katherine, Schier, Alexander
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blastula/metabolism*
- Body Patterning*/genetics
- Gene Expression Regulation, Developmental
- Left-Right Determination Factors/genetics
- Left-Right Determination Factors/metabolism*
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Signal Transduction
- Time Factors
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30061678 Full text @ Nat. Cell Biol.
Citation
Almuedo-Castillo, M., Bläßle, A., Mörsdorf, D., Marcon, L., Soh, G.H., Rogers, K.W., Schier, A.F., Müller, P. (2018) Scale-invariant patterning by size-dependent inhibition of Nodal signalling. Nature cell biology. 20(9):1032-1042.
Abstract
Individuals can vary substantially in size, but the proportions of their body plans are often maintained. We generated smaller zebrafish by removing 30% of their cells at the blastula stages and found that these embryos developed into normally patterned individuals. Strikingly, the proportions of all germ layers adjusted to the new embryo size within 2 hours after cell removal. As Nodal-Lefty signalling controls germ-layer patterning, we performed a computational screen for scale-invariant models of this activator-inhibitor system. This analysis predicted that the concentration of the highly diffusive inhibitor Lefty increases in smaller embryos, leading to a decreased Nodal activity range and contracted germ-layer dimensions. In vivo studies confirmed that Lefty concentration increased in smaller embryos, and embryos with reduced Lefty levels or with diffusion-hindered Lefty failed to scale their tissue proportions. These results reveal that size-dependent inhibition of Nodal signalling allows scale-invariant patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping