PUBLICATION
Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish
- Authors
- Vagionitis, S., Czopka, T.
- ID
- ZDB-PUB-180715-3
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1791: 25-35 (Chapter)
- Registered Authors
- Czopka, Tim, Vagionitis, Stavros
- Keywords
- Central nervous system, In vivo imaging, Myelin, Oligodendrocyte
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biomarkers
- Microscopy, Fluorescence
- Molecular Imaging*/instrumentation
- Molecular Imaging*/methods
- Myelin Sheath/metabolism*
- Neuroglia/metabolism*
- Time-Lapse Imaging*/instrumentation
- Time-Lapse Imaging*/methods
- Zebrafish
- PubMed
- 30006699 Full text @ Meth. Mol. Biol.
Citation
Vagionitis, S., Czopka, T. (2018) Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1791:25-35.
Abstract
In vivo time-lapse microscopy provides important information about the kinetics of cellular events and their control by interactions with neighboring cells. Here, we describe the generation and use of transgenic zebrafish to visualize dynamics of myelinating glia using cell type-specific expression and microscopy of genetically encoded fluorescent proteins. With this method, we are able to simultaneously separate and trace up to three different colors over time.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping